An S, Bleu T, Huang W, Hallmark O G, Coughlin S R, Goetzl E J
Department of Medicine, University of California, San Francisco 94143, USA.
FEBS Lett. 1997 Nov 17;417(3):279-82. doi: 10.1016/s0014-5793(97)01301-x.
The structural similarity of lysosphingolipids to lysophosphatidic acid (LPA) prompted a sequence-based search for lysosphingolipid receptors using cDNA sequence of the Edg2 human LPA receptor. Two closely related G protein-coupled receptors, rat H218 and human Edg3, are highly similar to Edg2. When overexpressed in Jurkat cells, H218 and Edg3 activated serum response element-driven transcriptional reporter gene in response to sphingosine 1-phosphate (S1P), dihydro-S1P and sphingosylphosphorylcholine, but not to LPA. H218 and Edg3 expressed in Xenopus oocytes conferred responsiveness to S1P and dihydro-S1P in agonist-triggered 45Ca2+ efflux. Therefore, H218 and Edg3 are functional receptors for S1P and perhaps other closely related lysosphingolipids.
溶血鞘脂与溶血磷脂酸（LPA）的结构相似性促使人们利用人LPA受体Edg2的cDNA序列，基于序列搜索溶血鞘脂受体。两个密切相关的G蛋白偶联受体，大鼠H218和人Edg3，与Edg2高度相似。当在Jurkat细胞中过表达时，H218和Edg3会响应鞘氨醇1-磷酸（S1P）、二氢-S1P和鞘氨醇磷酰胆碱激活血清反应元件驱动的转录报告基因，但对LPA无反应。在非洲爪蟾卵母细胞中表达的H218和Edg3在激动剂触发的45Ca2+外流中赋予了对S1P和二氢-S1P的反应性。因此，H218和Edg3是S1P以及可能其他密切相关的溶血鞘脂的功能性受体。
