Xi X P, Graf K, Goetze S, Hsueh W A, Law R E
University of California, Los Angeles, School of Medicine 90024, USA.
FEBS Lett. 1997 Nov 17;417(3):283-6. doi: 10.1016/s0014-5793(97)01303-3.
Insulin-stimulated DNA synthesis, MAP kinase (MAPK) activity and c-fos expression in vascular smooth muscle cells (VSMCs) was blocked by the MAPK inhibitor PD 98059. Regulation of c-fos expression by the transcription factor Elk-1 at the serum response element (SRE) is dependent on its phosphorylation by MAPK. PD 98059 also suppressed insulin-induced Elk-1 transcriptional activity through the SRE. These data show that MAPK plays a critical role in both insulin-mediated growth and Elk-1-dependent induction of c-fos in VSMCs.
丝裂原活化蛋白激酶（MAPK）抑制剂PD 98059可阻断胰岛素刺激的血管平滑肌细胞（VSMC）中的DNA合成、MAP激酶（MAPK）活性及c-fos表达。转录因子Elk-1在血清反应元件（SRE）处对c-fos表达的调控依赖于其被MAPK磷酸化。PD 98059还通过SRE抑制胰岛素诱导的Elk-1转录活性。这些数据表明，MAPK在VSMC中胰岛素介导的生长及Elk-1依赖的c-fos诱导过程中均起关键作用。
