Kream J, Hellman L, Rosenfeld R S
Steroids. 1976 Jun;27(6):727-39. doi: 10.1016/0039-128x(76)90133-1.
Details of a sensitive and specific radioimmunoassay for androsterone (1) and androsterone sulfate in plasma have been presented. Benzene extracts of plasma were chromatographed on alumina to isolate the androsterone fraction either (a) directly after extraction (A) or (b) after solvolysis (AS). Following treatment with rabbit anti-A-17-BSA, antibody bound steriod was precipitated by ammonium sulfate. Androsterone concentrations in normal male plasma averaged 57 +/- 24 (S.D.) ng/dl, range 35-135 ng/dl and for normal women, 44 +/- 21 (S.D.) ng/dl, range 18-98 ng/dl. Androsterone sulfate concentrations were: males 55 +/- 28 mug/dl (range 10-114 mug/dl); premenopausal females 52+/- 31 mug/dl (range 16-318 mug/dl).
已介绍了一种用于检测血浆中雄酮（1）和硫酸雄酮的灵敏且特异的放射免疫分析方法的详细信息。血浆的苯提取物在氧化铝上进行色谱分离，以分离雄酮部分，方法如下：（a）提取后直接分离（A）或（b）溶剂解后分离（AS）。用兔抗A-17-牛血清白蛋白处理后，结合抗体的类固醇通过硫酸铵沉淀。正常男性血浆中雄酮浓度平均为57±24（标准差）ng/dl，范围为35 - 135 ng/dl；正常女性为44±21（标准差）ng/dl，范围为18 - 98 ng/dl。硫酸雄酮浓度为：男性55±28 μg/dl（范围10 - 114 μg/dl）；绝经前女性52±31 μg/dl（范围16 - 318 μg/dl）。
