Marakusha B I, Temezhnikova N D, Tartakovskił I S
Antibiot Khimioter. 1997;42(10):33-7.
Preliminary estimation of virulence in some antibiotic resistant mutants of Legionella pneumophila, Philadelphia 1 in various models of infection revealed its decrease in the mutants resistant to azlocillin, cefotaxime, fluoroquinolone LIB-80, neamine and streptomycin. Detailed investigation of the neamine resistant mutants showed that in relation to streptomycin susceptibility such mutants could be divided into 3 classes: susceptible to streptomycin, resistant to high concentrations of streptomycin and resistant to moderate concentrations of streptomycin. Part of mutants Nea(r)Strr and Nea(r)Strr500 and all mutants Nea(r)Strr100 proved to be less virulent with respect to guinea pigs and chick embryos. The study of the spectinomycin resistant mutants of Legionella spp. did not reveal any changes in the virulence which made it possible to suggest that the influence of the mutations in the ribosomal protein genes determining resistance to streptomycin and neamine on virulence of L. pneumophila was based on the interdependence of the mutant effect on the suppression and the influence on the virulence detected by us in S. flexneri, Y. pseudotuberculosis, L. monocytogenes and F. tularensis. The Legionella mutants Nea(r)Strr100 were characterized by significant protective activity and protected immunized guinea pigs when tested in a model of their aerogenic infection.
对嗜肺军团菌费城 1 型某些抗生素抗性突变体在各种感染模型中的毒力进行初步评估，结果显示，对阿洛西林、头孢噻肟、氟喹诺酮 LIB - 80、新霉素和链霉素耐药的突变体的毒力有所降低。对新霉素抗性突变体的详细研究表明，就链霉素敏感性而言，此类突变体可分为 3 类：对链霉素敏感、对高浓度链霉素耐药和对中等浓度链霉素耐药。部分 Nea(r)Strr 和 Nea(r)Strr500 突变体以及所有 Nea(r)Strr100 突变体对豚鼠和鸡胚的毒力较低。对嗜肺军团菌属壮观霉素抗性突变体的研究未发现毒力有任何变化，这表明决定对链霉素和新霉素耐药性的核糖体蛋白基因突变对嗜肺军团菌毒力的影响，是基于我们在弗氏志贺菌、假结核耶尔森菌、单核细胞增生李斯特菌和土拉弗朗西斯菌中检测到的突变对抑制作用的影响与对毒力的影响之间的相互依存关系。嗜肺军团菌 Nea(r)Strr100 突变体具有显著的保护活性，在气源性感染模型中对免疫豚鼠具有保护作用。
