[A microfluorimetric study of glycogen fractions in rat liver under different conditions of liver perfusion and animal nutrition].

A study was made of the amount of a labile and a stable glycogen fractions in the rat liver cells under various feeding regimes and different durations of liver perfusion. The amount of the glycogen fraction revealed after a 40 minutes' treatment in a Schiff type reagent--Auramine--SO2 was found most chaneable at hunger, at feeding with carbohydrate rich food and at liver perfusion. This fraction is removed from the cells after the treatment with cold trichloroacetic acid (TCA-fraction). The amount of the glycogen fraction revealed after a more prolonged treatment of cells (90 minutes) in Auramine--SO2 and extracted only with hot KOH (KOH fraction), is relatively stable. According to the cytochemical evidence, the TCA and KOH fraction contents in the rat liver cells reach 80--85 and 15--20%, resp. The cytochemical evidence provided obtained with the fluorescence PAS-reaction permits to consider as identic the glycogen fractions revealed with biochemical methods.