del Rio M R, DeFelipe J
Instituto Cajal (CSIC), Madrid, Spain.
J Chem Neuroanat. 1997 Oct;13(4):243-51. doi: 10.1016/s0891-0618(97)00050-1.
We have examined the distribution of double bouquet cell axons, immunocytochemically stained for the calcium-binding proteins calretinin and calbindin D-28k in the human temporal neocortex, in relation to bundles of myelinated axons (originating from pyramidal cells) and the colocalization of these calcium-binding proteins. The large number and regularity of distribution of double bouquet cell axons was clearly visualized in tangential sections from cortical layers III--V. In these sections, we estimated that the mean number +/- standard deviation of double bouquet cell axons per 10,000 microns2 was 11.65 +/- 0.44 with a mean diameter of 12.10 +/- 0.63 microns and a mean center-to-center spacing of 29.8 +/- 0.91 microns. These values are very similar to those previously reported in the monkey neocortex. The distribution of double bouquet cell axons was closely related to bundles of myelinated axons; there was overlapping with basically a one-to-one correspondence. Finally, double-label immunofluorescence experiments revealed that the vast majority of double bouquet cell axons immunoreactive for calbindin were also stained for calretinin. Since relatively few cell somata were double-labeled in the human temporal cortex, we concluded that double bouquet cells may represent a significant subpopulation of neurons that colocalize these calcium-binding proteins.
我们通过免疫细胞化学方法，研究了人类颞叶新皮质中双重花束状细胞轴突的分布情况，这些轴突针对钙结合蛋白钙视网膜蛋白和钙结合蛋白D-28k进行了染色，同时研究了其与有髓轴突束（源自锥体细胞）的关系以及这些钙结合蛋白的共定位情况。在皮质III-V层的切线切片中，双重花束状细胞轴突分布的数量众多及规律性清晰可见。在这些切片中，我们估计每10,000平方微米双重花束状细胞轴突的平均数量±标准差为11.65±0.44，平均直径为12.10±0.63微米，平均中心间距为29.8±0.91微米。这些数值与先前在猴新皮质中报道的数值非常相似。双重花束状细胞轴突的分布与有髓轴突束密切相关；两者基本一一对应且存在重叠。最后，双重标记免疫荧光实验显示，绝大多数对钙结合蛋白呈免疫反应的双重花束状细胞轴突也被钙视网膜蛋白染色。由于在人类颞叶皮质中相对较少的细胞体被双重标记，我们得出结论，双重花束状细胞可能代表了共定位这些钙结合蛋白的神经元的一个重要亚群。
