采用柱前荧光衍生化高效液相色谱法测定微藻中的游离氨基酸。
Determination of free amino acids in microalgae by high-performance liquid chromatography using pre-column fluorescence derivatization.
作者信息
Betancort Rodríguez J R, García Reina G, Santana Rodríguez J J
机构信息
Department of Chemistry, Faculty of Marine Sciences, University of Las Palmas de Gran Canaria, Spain.
出版信息
Biomed Chromatogr. 1997 Nov-Dec;11(6):335-6. doi: 10.1002/(SICI)1099-0801(199711)11:6<335::AID-BMC684>3.0.CO;2-T.
An analytical method for a rapid reverse-phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r > 0.99) in the range 100-1000 nM. The proposed method was applied to the determination of free amino acids in two microalgae species (Chlorella pyrenoidosa and Chlamydomonas rinhardtii). The principal aminoacids found were alanine, arginine leucine, serine and glutamic acid.
介绍了一种用于氨基酸快速反相液相色谱分析的方法。总分析时间为19分钟。测量峰面积的平均相对偏差低于10%，并且对于OPA衍生物，荧光响应在100 - 1000 nM范围内与浓度呈线性关系（r > 0.99）。所提出的方法应用于两种微藻（小球藻和莱茵衣藻）中游离氨基酸的测定。检测到的主要氨基酸有丙氨酸、精氨酸、亮氨酸、丝氨酸和谷氨酸。
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