Mills P C, Ng J C, Kramer H, Auer D E
Department of Veterinary Pathology, University of Queensland, St Lucia, Australia.
Equine Vet J. 1997 Nov;29(6):483-6. doi: 10.1111/j.2042-3306.1997.tb03163.x.
Five clinically healthy Thoroughbred geldings were injected with Freund's adjuvant 3 times to induce a chronic inflammatory response. Blood was collected at various times before and after adjuvant administration. Clinical responses (rectal temperature and general demeanor) were also monitored. Adjuvant injection induced increases in rectal temperature and plasma fibrinogen concentration (maximum levels measured were mean +/- s.d. 39.7 +/- 0.5 degrees C and 8.2 +/- 0.3 g/l, respectively), indicative of an inflammatory response. A mild clinical depression was also observed in the horses for 24 h after the first injection of adjuvant only. Plasma cortisol levels decreased significantly from control levels of mean +/- s.d. 187.7 +/- 24.3 nmol/l to a minimum of 80.2 +/- 22.1 nmol/l (P < 0.01) 9 days after the first injection of adjuvant. Conversely, plasma insulin levels increased after the first injection of adjuvant to a maximum (96.7 +/- 15.2 iu/ml; P < 0.01) 12 days later, while plasma glucose concentrations tended to decline. A control group of horses to rule out contemporary environmental influences on the physiological and biochemical indices measured was not included in this study. The results show that chronic inflammation in the horse depressed resting plasma cortisol concentrations.
五匹临床健康的纯种马被注射弗氏佐剂3次以诱导慢性炎症反应。在佐剂给药前后的不同时间采集血液。还监测了临床反应（直肠温度和总体神态）。佐剂注射导致直肠温度和血浆纤维蛋白原浓度升高（测量的最高水平分别为平均±标准差39.7±0.5℃和8.2±0.3g/l），表明存在炎症反应。仅在首次注射佐剂后24小时内，马匹还出现了轻度的临床抑郁。首次注射佐剂9天后，血浆皮质醇水平从平均±标准差187.7±24.3nmol/l的对照水平显著下降至最低80.2±22.1nmol/l（P<0.01）。相反，首次注射佐剂后血浆胰岛素水平在12天后升高至最高值（96.7±15.2iu/ml；P<0.01），而血浆葡萄糖浓度则趋于下降。本研究未纳入用于排除当代环境对所测生理和生化指标影响的对照组马匹。结果表明，马匹的慢性炎症会降低静息血浆皮质醇浓度。
