Ross E V, Romero R, Kollias N, Crum C, Anderson R R
Department of Dermatology, Harvard Medical School, Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Boston, USA.
Br J Dermatol. 1997 Nov;137(5):736-42.
To examine the potential of using photodynamic therapy (PDT) in condylomata, we studied the distribution and kinetics of protoporphyrin IX (PpIX) formation in condylomata acuminata and adjacent normal skin after topical application of 5-aminolaevulinic acid (ALA). PpIX fluorescence spectra were measured hourly in vivo after ALA application. After gross fluorescence imaging, the lesions were biopsied, and fluorescence microscopy was performed. All three PpIX fluorescence detection modalities suggested selectivity of PpIX formation in condylomata after topical ALA application. In 17 of 25 condylomata, there was significantly greater fluorescence compared with adjacent normal skin. The greatest lesional to normal skin fluorescence ratios occurred after 2 h. The most likely mechanism for increased lesional PpIX formation in condylomata is enhanced stratum corneum permeability. Based on our results, ALA/PDT is a potential field therapy for condylomata. PpIX fluorescence imaging after ALA application may also be useful for localizing condylomata prior to treatment.
为了研究光动力疗法（PDT）在尖锐湿疣治疗中的潜力，我们研究了外用5-氨基乙酰丙酸（ALA）后尖锐湿疣及相邻正常皮肤中原卟啉IX（PpIX）的形成分布及动力学。外用ALA后，每小时在体内测量PpIX荧光光谱。在进行整体荧光成像后，对病变组织进行活检，并进行荧光显微镜检查。所有三种PpIX荧光检测方式均表明外用ALA后尖锐湿疣中PpIX的形成具有选择性。25例尖锐湿疣中有17例与相邻正常皮肤相比荧光明显增强。病变组织与正常皮肤的荧光比值在2小时后最高。尖锐湿疣中病变组织PpIX形成增加的最可能机制是角质层通透性增强。基于我们的研究结果，ALA/PDT是一种治疗尖锐湿疣的潜在局部治疗方法。外用ALA后的PpIX荧光成像在治疗前定位尖锐湿疣方面可能也有用。
