Stefanaki I M, Georgiou S, Themelis G C, Vazgiouraki E M, Tosca A D
Department of Dermatology, Heraklion University General Hospital, Heraklion 71003, Crete, Greece.
Br J Dermatol. 2003 Nov;149(5):972-6. doi: 10.1111/j.1365-2133.2003.05553.x.
Topical application of 5-aminolaevulinic acid (ALA) to condylomata acuminata leads to accumulation of protoporphyrin IX (PpIX); therefore ALA-induced photodynamic therapy (ALA-PDT) appears to be a potential treatment.
To investigate in vivo the PpIX fluorescence time course after topical application of ALA in order to determine the optimal time for irradiation, and to assess the efficacy of subsequently performed ALA-PDT.
Fluorescence kinetics was studied in 12 male patients with condylomata acuminata. Confirmation of diagnosis was established with conventional histology and polymerase chain reaction. Lesions were treated with 20% ALA and irradiated at the optimal time with a dose of 70 J cm-2 or 100 J cm-2 light. An additional session with 100 J cm-2 was administered 1 week later to lesions that persisted.
The in vivo study of fluorescence kinetics indicated that the optimal time for irradiation varied among patients from 6 to 11 h. The overall cure rate was 72.9%, 12 months after treatment.
Topical ALA-PDT is a potentially effective treatment for condylomata acuminata.
将5-氨基酮戊酸（ALA）局部应用于尖锐湿疣可导致原卟啉IX（PpIX）蓄积；因此，ALA诱导的光动力疗法（ALA-PDT）似乎是一种有潜力的治疗方法。
在体内研究ALA局部应用后的PpIX荧光时间进程，以确定最佳照射时间，并评估随后进行的ALA-PDT的疗效。
对12例男性尖锐湿疣患者进行荧光动力学研究。通过传统组织学和聚合酶链反应确诊。病变部位用20%的ALA治疗，并在最佳时间以70 J/cm²或100 J/cm²的剂量进行光照。1周后，对持续存在的病变部位再给予100 J/cm²的照射。
荧光动力学的体内研究表明，患者的最佳照射时间在6至11小时之间有所不同。治疗12个月后的总体治愈率为72.9%。
局部ALA-PDT是尖锐湿疣的一种潜在有效治疗方法。
