Shukoliukov S A, Lapshina A A, Kazantsev S I
Zh Evol Biokhim Fiziol. 1976 May-Jun;12(3):233-9.
Kinetic analysis, electrophoresis and selective inactivation were used in studies of isozymic composition of LDH in the retina, cardiac and skeletal muscles in 8 species of marine teleost fishes. In the retina, the number of isoenzymes in all the fishes studied varied from 2 to 7, the predominant ones being B4 and E4. The isoenzyme E4 was found only in the retina of Hexagrammus octogrammus, Pleurogrammus monopterygius, Liopsetts pannifasciata, Limanda yokohamae, Eleginus gracilis, Therarga chalcogramma. In two last species, the electric charge of E4, B4 and A4 is opposite to that of the corresponding isoenzyme in terrestrial animals, resulting in opposite migration of these isoenzymes during electrophoresis. In the retina of fishes containing the isoenzyme E4, double heterotetramers B1E3, B2E2, B3E1 were also found. The retina in Leuciscus brandti, Clupea harengus harengus does not contain the isoenzyme E4. Investigation of the distribution pattern of the isoenzymes in cellular fractions of the retina in P. monopterygius revealed that hyaloplasm and nuclei contain A4, B4, B3E1, E2B2, E3B1 and E4, which corresponds to the situation found after the extraction of LDH from the whole retina tissue. In the mitochondria B4 and E4 were found, while in the outer segments--only B4. Skeletal muscles in all the fishes studied differ by the predominance of the isoenzyme A4 which is present as a sole component (P. monopterygius, H. octogrammus, L. pannifasciata, L yokohamae, L. brandti) or together with B4 (T. chalcogramma, C. harengus harengus). In the cardiac muscles of four species of fishes (except L. yokohamae, L. pannifasciata, H. octogrammus, P. monopterygius), the predominant isoenzyme was found to be B4, although the total number and the ratio of the isoenzymes varied: A4, A2B2, B4 (E. gracilis), A4B4 (T. chalcogramma, C. harengus harengus), A4, A3B1, A2B2, A1B3, B4 (L. brandti). The LDH in the heart of L. yokohamae, L. pannifasciata, H. octogrammus, P. monopterygius has only one isoenzyme A4.
运用动力学分析、电泳和选择性失活方法，对8种海洋硬骨鱼类视网膜、心肌和骨骼肌中乳酸脱氢酶（LDH）的同工酶组成进行了研究。在视网膜中，所有研究鱼类的同工酶数量从2种到7种不等，主要的同工酶是B4和E4。同工酶E4仅在八线六线鱼、单鳍多线鱼、斑鳍红娘鱼、横滨黄盖鲽、细体油胡瓜鱼、铜带眶棘鲈的视网膜中发现。在最后两种鱼类中，E4、B4和A4的电荷与陆生动物相应同工酶的电荷相反，导致这些同工酶在电泳过程中向相反方向迁移。在含有同工酶E4的鱼类视网膜中，还发现了双杂合四聚体B1E3、B2E2、B3E1。勃氏雅罗鱼、大西洋鲱的视网膜中不含同工酶E4。对单鳍多线鱼视网膜细胞组分中同工酶分布模式的研究表明，透明质和细胞核中含有A4、B4、B3E1、E2B2、E3B1和E4，这与从整个视网膜组织中提取LDH后的情况一致。在线粒体中发现了B4和E4，而在外段仅发现了B4。所有研究鱼类的骨骼肌中，同工酶A4占优势，A4是唯一的组分（单鳍多线鱼、八线六线鱼、斑鳍红娘鱼、横滨黄盖鲽、勃氏雅罗鱼），或者与B4一起存在（铜带眶棘鲈、大西洋鲱）。在四种鱼类的心肌中（除横滨黄盖鲽、斑鳍红娘鱼、八线六线鱼、单鳍多线鱼外），主要的同工酶是B4，尽管同工酶的总数和比例有所不同：A4、A2B2、B4（细体油胡瓜鱼），A4B4（铜带眶棘鲈、大西洋鲱），A4、A3B1、A2B2、A1B3、B4（勃氏雅罗鱼）。横滨黄盖鲽、斑鳍红娘鱼、八线六线鱼、单鳍多线鱼心脏中的LDH只有一种同工酶A4。
