Pointner H, Flegel U
Acta Hepatogastroenterol (Stuttg). 1976 Jun;23(3):201-9.
Gastrin was extracted from normal porcine pancreas by the method used by Gregory and Tracy for the extraction of gastrin from the antrum. Radioimmuno-assay was used for identification at all stages of the extraction procedure. Gastrin of pancreatic origin was eluted from a sephadex G 50 and a DEAE-collulose column at the soma position as porcine antral gastrin. In disc electrophoresis (7.5% polyacrylamide in tris-glycin buffer) we found a single band which migrated the same distance from the origin as reference gastrin (synthetic human gastrin I). Densitometircally there was also a single band. The eluate of this electrophoretic band produced an increase in gastric acid secretion after intravenous injection in the rat. A dilution series was made from the extracted fraction and radioimmunologically tested with gastrin antibodies. The individual points of the dilution curve corresponded well with the standard calibration curve of gastrin. A certain discrepancy between the small amount of gastrin found by radioimmunoassay (500 pg/g fresh pancreas) and biochemical and biological findings could probably be explained by a different sensitivity of the gastrin antibody of the radioimmunological system to pancreatic gastrin. Our results prove that there is gastrin in the normal porcine pancreas.
采用格雷戈里和特雷西从胃窦提取胃泌素的方法，从正常猪胰腺中提取胃泌素。在提取过程的各个阶段都采用放射免疫分析法进行鉴定。胰腺来源的胃泌素从葡聚糖凝胶G 50和二乙氨基乙基纤维素柱上洗脱下来，其位置与猪胃窦胃泌素相同。在圆盘电泳（在三羟甲基氨基甲烷-甘氨酸缓冲液中的7.5%聚丙烯酰胺）中，我们发现有一条单一的条带，它从原点迁移的距离与对照胃泌素（合成人胃泌素I）相同。用密度计测量也只有一条单一的条带。这条电泳条带的洗脱液在给大鼠静脉注射后，可使胃酸分泌增加。从提取的组分中进行稀释系列，并与胃泌素抗体进行放射免疫检测。稀释曲线的各个点与胃泌素的标准校准曲线吻合良好。放射免疫分析法检测到的胃泌素量较少（500 pg/g新鲜胰腺），与生化和生物学结果之间存在一定差异，这可能是由于放射免疫系统的胃泌素抗体对胰腺胃泌素的敏感性不同所致。我们的结果证明正常猪胰腺中存在胃泌素。
