Kaufmann O, Georgi T, Dietel M
Institute of Pathology, Charité Hospital, Humboldt University Berlin, Germany.
Hum Pathol. 1997 Dec;28(12):1373-8. doi: 10.1016/s0046-8177(97)90226-4.
CD56 is immunohistochemically detectable in virtually all small cell carcinomas on frozen sections. The authors retrospectively tested the usefulness of the monoclonal antibody 123C3 against CD56 to differentiate pulmonary and extrapulmonary small cell carcinomas from nonneuroendocrine non-small cell carcinomas by paraffin-section immunohistochemistry after antigen retrieval. The study included 70 small cell carcinomas and 344 primary and metastatic nonneuroendocrine carcinomas of various primary sites. The staining results were compared with specific neuroendocrine markers (CD57, Chromogranin A, Synaptophysin). The monoclonal antibody 123C3 diffusely stained most small cell carcinomas with a strong membranous pattern (sensitivity: 0.99). The staining intensity was not diminished in areas with crush artifacts or after decalcification. The neuroendocrine markers had a combined sensitivity of only 0.44 for small cell carcinomas. With regard to nonneuroendocrine carcinomas, the 123C3 antibody stained 7 of 28 ovarian carcinomas, 6 of 30 renal cell carcinomas, 2 of 10 endometrial carcinomas, two of three nonneuroendocrine large cell carcinomas of the lung, 1 of 38 adenocarcinomas, and 4 of 52 squamous cell carcinomas of the lung. Urothelial carcinomas, hepatocellular carcinomas, squamous carcinomas of the head/neck and cervix uteri, as well as adenocarcinomas of the breast, stomach, colon, pancreas, and prostate, showed no immunoreactivity for CD56. The specificities of 123C3 and the combined neuroendocrine markers for small cell carcinomas were 0.94 and 0.95, respectively. The authors conclude that monoclonal antibody 123C3 might be useful for the immunohistochemical differentiation of small cell carcinomas from nonneuroendocrine carcinomas on paraffin sections, especially in small and crushed biopsy specimens.
在冰冻切片上，几乎所有小细胞癌均可通过免疫组织化学检测到CD56。作者回顾性地测试了抗CD56单克隆抗体123C3在抗原修复后通过石蜡切片免疫组织化学区分肺和肺外小细胞癌与非神经内分泌非小细胞癌的效用。该研究纳入了70例小细胞癌以及344例来自不同原发部位的原发性和转移性非神经内分泌癌。将染色结果与特定神经内分泌标志物（CD57、嗜铬粒蛋白A、突触素）进行比较。单克隆抗体123C3以强膜性模式弥漫性地染色了大多数小细胞癌（敏感性：0.99）。在有挤压伪像的区域或脱钙后，染色强度并未减弱。神经内分泌标志物对小细胞癌的联合敏感性仅为0.44。对于非神经内分泌癌，123C3抗体染色了28例卵巢癌中的7例、30例肾细胞癌中的6例、10例子宫内膜癌中的2例、3例肺非神经内分泌大细胞癌中的2例、38例腺癌中的1例以及52例肺鳞状细胞癌中的4例。尿路上皮癌、肝细胞癌、头颈部和子宫颈鳞状癌以及乳腺、胃、结肠、胰腺和前列腺腺癌对CD56无免疫反应。123C3和联合神经内分泌标志物对小细胞癌的特异性分别为0.94和0.95。作者得出结论，单克隆抗体123C3可能有助于在石蜡切片上通过免疫组织化学区分小细胞癌与非神经内分泌癌，尤其是在小的和挤压的活检标本中。
