Mooi W J, Wagenaar S S, Schol D, Hilgers J
Division of Tumour Biology, Netherlands Cancer Institute, Amsterdam.
Mol Cell Probes. 1988 Mar;2(1):31-7. doi: 10.1016/0890-8508(88)90041-2.
Monoclonal antibody 123C3, raised in The Netherlands Cancer Institute against a membrane preparation of small cell lung carcinoma, was tested on a series of 358 surgically resected primary epithelial lung tumours of various types. 123C3 recognized all small cell carcinomas and carcinoids tested (total: 30 tumours), but also stained a minority of various types of non-small cell lung carcinomas and almost all bronchial gland tumours investigated. Staining for 123C3 in squamous carcinomas or adenocarcinomas, when present, was usually focal and weak or moderately strong, whereas staining of small cell carcinomas and carcinoids was strong and present throughout the tumour. Our results indicate that immunostaining for 123C3 may be of potential interest for the histological typing of lung tumours, particularly when staining results are either strongly positive or completely negative. The positivity found in a fraction of non-small cell lung carcinomas is yet another illustration of the fact that the major types of lung tumours cannot be strictly separated, and that overlaps in differentiation states and antigenic make-up exist between different histological types of lung tumours.
在荷兰癌症研究所制备的、针对小细胞肺癌膜制剂的单克隆抗体123C3，在一系列358例经手术切除的不同类型原发性上皮性肺肿瘤上进行了检测。123C3识别所有检测的小细胞癌和类癌（共30个肿瘤），但也对少数不同类型的非小细胞肺癌以及几乎所有研究的支气管腺肿瘤进行了染色。鳞状细胞癌或腺癌中123C3染色若存在，通常呈局灶性，弱阳性或中度强阳性，而小细胞癌和类癌的染色则为强阳性且遍布整个肿瘤。我们的结果表明，123C3免疫染色可能对肺肿瘤的组织学分型具有潜在意义，特别是当染色结果为强阳性或完全阴性时。在一部分非小细胞肺癌中发现的阳性结果再次说明了肺肿瘤的主要类型不能严格区分，不同组织学类型的肺肿瘤在分化状态和抗原组成上存在重叠这一事实。
