一种使用气相色谱和质谱法测量炎症渗出物中极少量前列腺素的方法。
A method for the measurement of ultra-low amounts of prostaglandins in inflammatory exudates using gas chromatography and mass spectrometry.
作者信息
Wilson B W, Snedden W, Tham K T, Willoughby D A
出版信息
Agents Actions. 1976 Feb;6(1-3):176-9. doi: 10.1007/BF01972204.
PMID:941791
Abstract
A GC-MS assay for PGE2 and PGF2alpha has been described. The method is highly specific and sensitive down to the sub-picogram level. The assay has been used to measure the time dependence of PG concentration in carrageenan-induced inflammatory exudates and the results compared with a bioassay. Estimates of the extraction and purification yield of PGs from exudates have also been obtained.
摘要
已描述了一种用于检测前列腺素E2（PGE2）和前列腺素F2α（PGF2α）的气相色谱-质谱联用（GC-MS）分析方法。该方法具有高度特异性，灵敏度可达亚皮克水平。该分析方法已用于测量角叉菜胶诱导的炎性渗出物中前列腺素浓度的时间依赖性，并将结果与生物测定法进行比较。还获得了从渗出物中提取和纯化前列腺素的产率估计值。
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