使用高效液相色谱法和气相色谱-质谱联用技术测定血浆中PGE2和PGF2α的15-酮-13,14-二氢代谢物。

Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2alpha in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry.

作者信息

Hubbard W C, Watson J T

出版信息

Prostaglandins. 1976 Jul;12(1):21-35. doi: 10.1016/s0090-6980(76)80004-4.

Abstract

A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF2alpha in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF2alpha into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13, 14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF2alpha found in peripheral plasma are 33 +/- 10 pg/ml (SD; n=6) and 40 +/- 16 pg/ml (SD; n=6), respectively.

摘要

本文描述了一种测量人外周血血浆中PGE2和PGF2α的15-酮-13,14-二氢代谢物的方法。该方法包括通过高效液相色谱法进行纯化，然后以代谢物的四氘代类似物作为内标，采用气相色谱-质谱联用技术测定其含量。血浆中这些代谢物的水平被认为比相应的初级前列腺素水平更能合理地反映PGE2和PGF2α进入外周血的情况。在外周血浆中发现的15-酮-13,14-二氢-PGE2和15-酮-13,14-二氢-PGF2α的内源性水平分别为33±10 pg/ml（标准差；n = 6）和40±16 pg/ml（标准差；n = 6）。