Singh M V, Bhatnagar R, Price C J, Malhotra S K
Department of Biology, Vanderbilt University, Nashville, Tennessee 37235, USA.
Cytobios. 1997;89(358-359):209-25.
The relationship between growth properties and gap junctional communication properties of two glioma cell lines, 9L and C6, was compared. Using microinjection of a gap junction permeable fluorescent dye, it was demonstrated that 9L cells were extensively dye coupled through gap junctions, whereas C6 cells had low levels of dye coupling between the cells. More gap junctions were observed between 9L cells than between C6 cells both by electron microscopy and immunofluorescent labelling with specific antibodies. Immunoblotting and immunofluorescence labelling of cellular proteins from 9L and C6 cells also showed differences in the Cx43 connexin contents of these cells, reflecting the differences in their gap junctional communication. Northern blot analyses confirmed that the level of Cx43 mRNA was higher in 9L than in C6 cells. In spite of these differences in gap junctions, the two cell lines did not differ in their growth rates in cultures. These results suggest a lack of direct correlation between gap junctional communication and cell growth regulation in these two glioma cell lines.
比较了两种胶质瘤细胞系9L和C6的生长特性与间隙连接通讯特性之间的关系。通过显微注射一种可透过间隙连接的荧光染料，结果表明9L细胞通过间隙连接广泛地进行染料偶联，而C6细胞之间的染料偶联水平较低。通过电子显微镜以及用特异性抗体进行免疫荧光标记发现，9L细胞之间的间隙连接比C6细胞之间更多。对9L和C6细胞的细胞蛋白进行免疫印迹和免疫荧光标记也显示，这些细胞中Cx43连接蛋白的含量存在差异，这反映了它们间隙连接通讯的差异。Northern印迹分析证实，9L细胞中Cx43 mRNA的水平高于C6细胞。尽管间隙连接存在这些差异，但这两种细胞系在培养中的生长速率并无差异。这些结果表明，在这两种胶质瘤细胞系中，间隙连接通讯与细胞生长调节之间缺乏直接相关性。
