Gunnlaugsdóttir B, Gudmundsdóttir B K
Institute for Experimental Pathology, University of Iceland, Keldur, Reykjavík, Iceland.
J Appl Microbiol. 1997 Nov;83(5):542-51. doi: 10.1046/j.1365-2672.1997.00247.x.
The pathogenicity of extracellular products (ECPs) from 24 atypical Aeromonas salmonicida strains was studied with respect to: lethality in Atlantic salmon, pathogenic effect in muscle, haemolytic activity, cytotoxicity in two fish cell lines and proteolytic activities. Furthermore, the relationship between lethality of ECPs and mortality caused by bacterial challenge was examined. Correlation was demonstrated between the pathogenic properties and proteolytic activities of the ECPs. Cytolytic (GCAT) activity comparable with that of the typical reference strain used (NCMB 1102) was not detected in ECPs of any of the atypical strain tested. An extracellular metallo-caseinase, AsaP1, was linked with lethal toxicity and a strong pathogenic effect. Furuncular-like lesions were produced by ECPs containing AsaP1 activity. One strain produced a lethal toxin which was neither caseinolytic nor with GCAT comparable activity. The examined atypical strains form at least three distinct groups based on different virulence mechanisms and extracellular proteases.
对24株非典型杀鲑气单胞菌菌株的胞外产物（ECPs）的致病性进行了研究，涉及以下方面：对大西洋鲑的致死性、对肌肉的致病作用、溶血活性、对两种鱼类细胞系的细胞毒性以及蛋白水解活性。此外，还研究了ECPs的致死性与细菌攻击所致死亡率之间的关系。结果表明，ECPs的致病特性与蛋白水解活性之间存在相关性。在所测试的任何非典型菌株的ECPs中，均未检测到与所用典型参考菌株（NCMB 1102）相当的溶细胞（GCAT）活性。一种细胞外金属酪蛋白酶AsaP1与致死毒性和强烈的致病作用有关。含有AsaP1活性的ECPs可产生疖样病变。一株菌株产生了一种致死毒素，该毒素既无酪蛋白水解活性，也无与GCAT相当的活性。基于不同的毒力机制和细胞外蛋白酶，所检测的非典型菌株至少形成三个不同的组。
