Vázquez J A, Docasal S F, Mirón J, González M P, Murado M A
Instituto de Investigacións Mariñas (CSIC), r/Eduardo Cabello, 6 Vigo, 36208, Galicia, Spain.
J Ind Microbiol Biotechnol. 2006 Aug;33(8):661-8. doi: 10.1007/s10295-006-0096-1. Epub 2006 Feb 24.
A comparative study was carried out on the growth and production of alkaline proteases by two Vibrio species using different marine peptones from fish viscera residues. The bacteria tested, Vibrio anguillarum and Vibrio splendidus, are producers of high levels of proteolytic enzymes which act as factors of virulence in fish cultures, causing high mortality rates. The kinetic assays and subsequent comparison with the parameters obtained from the adjustment to various mathematical models, highlighted the potential interest of the media formulated, for their possible production on an industrial scale, particularly the production of proteases by V. anguillarum growing in rainbow trout and squid peptones.
利用来自鱼内脏残渣的不同海洋蛋白胨,对两种弧菌属细菌产生碱性蛋白酶的生长和产量进行了比较研究。受试细菌鳗弧菌和灿烂弧菌是高水平蛋白水解酶的产生者,这些酶在鱼类养殖中作为毒力因子,导致高死亡率。动力学分析以及随后与通过拟合各种数学模型获得的参数进行比较,突出了所配制培养基在工业规模生产方面的潜在价值,特别是鳗弧菌在虹鳟鱼和鱿鱼蛋白胨中生长时产生蛋白酶的情况。