Borin S, Daffonchio D, Sorlini C
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche (DISTAM), Università degli Studi, Milano, Italy.
FEMS Microbiol Lett. 1997 Dec 1;157(1):87-93. doi: 10.1111/j.1574-6968.1997.tb12757.x.
The suitability of tDNA-PCR fingerprinting to identify species of the genus Bacillus was tested on 75 strains. Strains belonging to the same species or the same phylogenetic cluster were correctly grouped. Among B. stearothermophilus strains, different pattern types were found. This could be due to the unclear taxonomic situation of these strains, rather than to a failure of the tDNA-PCR. Single strand conformation polymorphism (SSCP) of the PCR products allowed species discrimination within the 'B. subtilis group', but not within the 'B. cereus group'. The tDNA-PCR, alone or coupled with SSCP analysis, is useful to address Bacillus species identification, particularly for those species which are not phylogenetically tightly clustered.
在75株菌株上测试了tDNA-PCR指纹图谱用于鉴定芽孢杆菌属物种的适用性。属于同一物种或同一系统发育簇的菌株被正确分组。在嗜热脂肪芽孢杆菌菌株中，发现了不同的模式类型。这可能是由于这些菌株的分类情况不明确，而不是tDNA-PCR失败所致。PCR产物的单链构象多态性（SSCP）可在“枯草芽孢杆菌组”内区分物种，但在“蜡样芽孢杆菌组”内则无法区分。单独的tDNA-PCR或与SSCP分析相结合，对于芽孢杆菌属物种的鉴定很有用，特别是对于那些在系统发育上没有紧密聚类的物种。
