Kügler S, Schüller S, Goebel W
Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität Würzburg, Germany.
FEMS Microbiol Lett. 1997 Dec 1;157(1):131-6. doi: 10.1111/j.1574-6968.1997.tb12763.x.
In this study we show that protein tyrosine kinases and also protein tyrosine phosphatases are involved in the uptake of Listeria monocytogenes by J774 macrophages to a different extent than in the uptake of inert latex beads. In addition, protein tyrosine kinases are necessary for the intracellular growth and survival of L. monocytogenes. The expression of the MAP kinase phosphatase MKP-1, a protein tyrosine phosphatase, is induced upon infection, and phagocytosis of L. monocytogenes by J774 cells overexpressing the MKP-1 protein is reduced compared to control cells. The decreased phagocytosis of L. monocytogenes as a result of the MKP-1 overexpression in J774 macrophages suggests that the activation of the MAP kinase(s) ERK-1 and/or ERK-2 is an essential requirement for the uptake of L. monocytogenes by J774 macrophages.
在本研究中，我们发现蛋白酪氨酸激酶和蛋白酪氨酸磷酸酶参与了J774巨噬细胞对单核细胞增生李斯特菌的摄取，其程度与对惰性乳胶珠的摄取不同。此外，蛋白酪氨酸激酶对于单核细胞增生李斯特菌在细胞内的生长和存活是必需的。感染后会诱导丝裂原活化蛋白激酶磷酸酶MKP-1（一种蛋白酪氨酸磷酸酶）的表达，与对照细胞相比，过表达MKP-1蛋白的J774细胞对单核细胞增生李斯特菌的吞噬作用降低。J774巨噬细胞中因MKP-1过表达导致的单核细胞增生李斯特菌吞噬作用降低表明，丝裂原活化蛋白激酶ERK-1和/或ERK-2的激活是J774巨噬细胞摄取单核细胞增生李斯特菌的必要条件。
