Weiglein I, Goebel W, Troppmair J, Rapp U R, Demuth A, Kuhn M
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften, Universität Würzburg, Germany.
FEMS Microbiol Lett. 1997 Mar 15;148(2):189-95. doi: 10.1111/j.1574-6968.1997.tb10287.x.
In this study we investigated the effect of Listeria monocytogenes infection on the activation of the Raf-MEK-MAP kinase pathway in eukaryotic host cells. HeLa cell infection with L. monocytogenes EGD resulted in a rapid, but transient, phosphorylation of the MAP kinases erk-1 and erk-2, a transient phosphorylation of the MAP kinase kinase MEK-1, and a transient activation of the MAP kinase kinase kinase Raf. In parallel to the transient phosphorylation of the MAP kinases, we detected induced expression of the MAP kinase phosphatase MKP-1. Additionally we present evidence that listeriolysin O is the inducing agent for activation of the Raf-MEK-MAP kinase pathway.
在本研究中，我们调查了单核细胞增生李斯特菌感染对真核宿主细胞中Raf-MEK-MAP激酶途径激活的影响。用单核细胞增生李斯特菌EGD感染HeLa细胞导致丝裂原活化蛋白激酶erk-1和erk-2迅速但短暂的磷酸化、丝裂原活化蛋白激酶激酶MEK-1的短暂磷酸化以及丝裂原活化蛋白激酶激酶激酶Raf的短暂激活。与丝裂原活化蛋白激酶的短暂磷酸化同时，我们检测到丝裂原活化蛋白激酶磷酸酶MKP-1的诱导表达。此外，我们提供证据表明，李斯特菌溶血素O是Raf-MEK-MAP激酶途径激活的诱导剂。
