家蚕中一种类水手元件的克隆与特性分析

Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori.

作者信息

Tomita S, Sohn B H, Tamura T

机构信息

Department of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science, Ibaraki, Japan.

出版信息

Genes Genet Syst. 1997 Aug;72(4):219-28. doi: 10.1266/ggs.72.219.

DOI:10.1266/ggs.72.219

PMID:9418262

Abstract

We have identified a mariner-like element (MLE) in the genome of the silkworm, Bombyx mori. The cloned MLE, desiqnated as BmMLE, contained an ORF that was interrupted by insertions, stop codons, and frameshifts. The complete ORF sequence was estimated by sequencing the PCR products. The hypothetical protein coded by this ORF showed 34% homology to the Mos1 element of Drosophila mauritiana, and is phylogenetically very close to MLE of Hyalophora cecropia. BmMLE is present in 80 to 100 copies in the B. mori genome. Many BmMLEs with different lengths were identified by Southern blot analysis and LA PCR of the genomic DNA. The distribution of these BmMLEs differed in geographic races.

摘要

我们在家蚕基因组中鉴定出一种类水手元件（MLE）。克隆得到的MLE，命名为BmMLE，包含一个被插入序列、终止密码子和移码突变打断的开放阅读框（ORF）。通过对PCR产物进行测序来估计完整的ORF序列。由该ORF编码的假定蛋白与毛里求斯果蝇的Mos1元件具有34%的同源性，并且在系统发育上与天蚕的MLE非常接近。BmMLE在家蚕基因组中以80到100个拷贝存在。通过对基因组DNA进行Southern杂交分析和连接介导的PCR（LA PCR）鉴定出许多不同长度的BmMLE。这些BmMLE的分布在地理品系中有所不同。