Robertson H M, Asplund M L
Department of Entomology, University of Illinos, Urbana 61801, USA.
Insect Biochem Mol Biol. 1996 Sep-Oct;26(8-9):945-54. doi: 10.1016/s0965-1748(96)00061-6.
We describe a transposable element, called Bmmar1, from the genome of the silkworm moth, Bombyx mori. This element has features of the Tc1-mariner superfamily of transposable elements. Bmmar1 was first detected as a fragment in the 5' region of the larval serum protein (BmLSP) gene. Six genomic clones characterized each differed from a consensus sequence by 3-5 insertions and deletions, as well as an average of 2.3% in nucleotide sequence. The genome contains approximately 2400 copies of Bmmar1. Maximum parsimony phylogenetic analysis of the relationship of Bmmar1 and other members of the Tc1-mariner superfamily, based on their encoded transposase amino acid sequences, indicates that it represents a basal lineage of the mariner family. In particular Bmmar1 encodes a D,D37D motif thought to be the catalytic domain of mariner transposases. Bmmar1 considerably increases the known diversity of this widespread family of transposons. A new naming system is proposed for members of the family.
我们描述了一种来自家蚕（Bombyx mori）基因组的转座元件，称为Bmmar1。该元件具有转座元件Tc1-mariner超家族的特征。Bmmar1最初是在幼虫血清蛋白（BmLSP）基因的5'区域作为一个片段被检测到的。六个经鉴定的基因组克隆与共有序列相比，每个都有3 - 5个插入和缺失，以及平均2.3%的核苷酸序列差异。基因组中大约包含2400个Bmmar1拷贝。基于其编码的转座酶氨基酸序列，对Bmmar1与Tc1-mariner超家族其他成员关系进行的最大简约系统发育分析表明，它代表了mariner家族的一个基部谱系。特别是Bmmar1编码一个被认为是mariner转座酶催化结构域的D,D37D基序。Bmmar1极大地增加了这个广泛存在的转座子家族已知的多样性。本文为该家族成员提出了一种新的命名系统。
