青鳉（Oryzias latipes）肝脏mRNA的表达序列标签
Expressed sequence tags of medaka (Oryzias latipes) liver mRNA.
作者信息
Hirono I, Aoki T
机构信息
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
出版信息
Mol Mar Biol Biotechnol. 1997 Dec;6(4):345-50.
A medaka liver cDNA library was constructed in lambda ZAPII. The number of clones in this library is approximately 5 x 10(6). Three hundred sixty-one clones were randomly selected and, of these, 33 clones with over 1 kb were sequenced. These sequences were compared with GenBank and the dbEST (Re.96.0). Twenty-five clones of these 33 clones encoded 18 different genes and 2 different expressed sequence tags (ESTs). Sequences of 10 of the 18 clones had not previously been reported in fish. The codon usage of medaka genes is similar to that of Xenopus, mouse, and human genes, but not similar to that of yeast and Escherichia coli genes.
构建了一个青鳉肝脏cDNA文库，该文库采用λZAPII载体。此文库中的克隆数约为5×10⁶个。随机挑选了361个克隆，其中33个长度超过1 kb的克隆进行了测序。将这些序列与GenBank和dbEST（版本96.0）进行了比较。这33个克隆中的25个克隆编码18种不同的基因和2种不同的表达序列标签（EST）。这18个克隆中的10个克隆的序列此前在鱼类中未曾报道过。青鳉基因的密码子使用情况与非洲爪蟾、小鼠和人类基因的密码子使用情况相似，但与酵母和大肠杆菌基因的密码子使用情况不同。
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