对牙鲆（Paralichthys olivaceus）肝脏和脾脏中表达基因的一项调查。
A survey of expressed genes in Japanese flounder (Paralichthys olivaceus) liver and spleen.
作者信息
Inoue S, Nam B H, Hirono I, Aoki T
机构信息
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
出版信息
Mol Mar Biol Biotechnol. 1997 Dec;6(4):376-80.
Expressed sequence tags (ESTs) were obtained from Japanese flounder (Paralichthys olivaceus). We present the results of single-pass sequencing of 493 ESTs from 350 clones from liver cDNA and 57 ESTs from 41 clones from spleen cDNA. Sequences of the cDNA clones were compared with sequences in the GenBank database. Two hundred and two clones (51.7%) appeared to be completely unknown and are likely to represent newly described genes, whereas 189 clones (48.3%) were identified based on matches to sequences in the databases. Three of the unidentified sequences were isolated from both the liver and spleen cDNA libraries. However, there were no identical sequences between liver and spleen clones.
从牙鲆（Paralichthys olivaceus）中获得了表达序列标签（ESTs）。我们展示了对来自肝脏cDNA的350个克隆中的493个ESTs以及来自脾脏cDNA的41个克隆中的57个ESTs进行单通道测序的结果。将cDNA克隆的序列与GenBank数据库中的序列进行了比较。202个克隆（51.7%）似乎完全未知，很可能代表新描述的基因，而189个克隆（48.3%）是根据与数据库中序列的匹配来鉴定的。有三个未鉴定的序列是从肝脏和脾脏cDNA文库中都分离得到的。然而，肝脏和脾脏克隆之间没有相同的序列。
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