Rybouchkin A V, Van der Straeten F, Quatacker J, De Sutter P, Dhont M
Department of Gynaecology and Obstetrics, University Hospital, Ghent, Belgium.
Fertil Steril. 1997 Dec;68(6):1144-7. doi: 10.1016/s0015-0282(97)00378-6.
To investigate a method of assisted activation of human oocytes for the treatment of infertility resulting from globozoospermia associated with deficient oocyte activation capacity.
The mouse oocyte activation test was used to analyze the oocyte activation capacity of the sperm cells of a patient with globozoospermia. Fresh donor human oocytes were used for determining the most appropriate procedure for oocyte activation.
Infertility Center, University Hospital of Ghent.
PATIENT(S): A couple with infertility resulting from globozoospermia.
INTERVENTION(S): Intracytoplasmic sperm injection, assisted oocyte activation, and embryo transfer.
MAIN OUTCOME MEASURE(S): Oocyte activation and fertilization rates, implantation, and pregnancy.
RESULT(S): Deficiency in oocyte activation capacity was found in the sperm of a patient with globozoospermia. This deficiency was proven by the mouse oocyte activation test and was confirmed further by lack of activation of human oocytes after simple sperm injection. Only human oocytes that were injected with sperm and subjected to calcium chloride and ionophore treatment underwent activation. Transfer of embryos obtained by this procedure of assisted oocyte activation resulted in an ongoing pregnancy.
CONCLUSION(S): Assisted oocyte activation of human oocytes is useful when globozoospermia is associated with absence of oocyte activation capacity in the sperm. These cases can be identified by the mouse oocyte activation test.
研究一种辅助激活人卵母细胞的方法，用于治疗因圆头精子症伴卵母细胞激活能力缺陷导致的不孕症。
采用小鼠卵母细胞激活试验分析一名圆头精子症患者精子细胞的卵母细胞激活能力。使用新鲜供体人卵母细胞确定最适宜的卵母细胞激活程序。
根特大学医院不孕症中心。
一对因圆头精子症导致不孕的夫妇。
卵胞浆内单精子注射、辅助卵母细胞激活及胚胎移植。
卵母细胞激活率和受精率、着床及妊娠情况。
在一名圆头精子症患者的精子中发现卵母细胞激活能力缺陷。小鼠卵母细胞激活试验证实了这种缺陷，单纯精子注射后人卵母细胞缺乏激活进一步证实了这一点。只有注射精子并经氯化钙和离子载体处理的人卵母细胞发生了激活。通过这种辅助卵母细胞激活程序获得的胚胎移植后实现了持续妊娠。
当圆头精子症伴精子中缺乏卵母细胞激活能力时，辅助激活人卵母细胞是有效的。这些病例可通过小鼠卵母细胞激活试验来识别。
