人类和大鼠卵巢卵泡中细胞凋亡的检测。
Detection of apoptosis in human and rat ovarian follicles.
作者信息
Piquette G N, Tilly J L, Prichard L E, Simón C, Polan M L
机构信息
Department of Gynecology and Obstetrics, Stanford University Medical Center, California, USA.
出版信息
J Soc Gynecol Investig. 1994 Oct-Dec;1(4):297-301. doi: 10.1177/107155769400100409.
OBJECTIVE
The purpose of this study was to determine whether cells acquired from individual human preovulatory follicles undergo apoptosis (physiologic cell death) and, if so, to correlate the degree of apoptosis with characteristics of the follicles or the oocytes derived from the follicles.
METHODS
We devised a sensitive nonradioactive method for detecting apoptotic DNA fragmentation in small numbers of cells derived from rat atretic follicles and follicular aspirates of patients undergoing assisted reproductive technologies.
RESULTS
Using this method, apoptotic DNA was detected in rat atretic follicles, with optimal detection at 10-100 ng. Furthermore, apoptotic DNA was detected in some, but not all individual human follicular aspirates from several patients, and was found in follicles that produced oocytes that fertilized and developed into embryos.
CONCLUSION
Apoptosis occurs in cells from human ovarian preovulatory follicles and may be a normal physiologic process of the follicle during luteinization.
目的
本研究旨在确定从个体人类排卵前卵泡获取的细胞是否会发生凋亡（生理性细胞死亡），如果发生凋亡，则将凋亡程度与卵泡或源自卵泡的卵母细胞的特征相关联。
方法
我们设计了一种灵敏的非放射性方法，用于检测来自大鼠闭锁卵泡和接受辅助生殖技术患者的卵泡抽吸物中的少量细胞的凋亡性DNA片段化。
结果
使用该方法，在大鼠闭锁卵泡中检测到凋亡性DNA，最佳检测量为10 - 100 ng。此外，在来自几名患者的部分（但并非全部）个体人类卵泡抽吸物中检测到凋亡性DNA，并且在产生的卵母细胞受精并发育成胚胎的卵泡中也发现了凋亡性DNA。
结论
凋亡发生在人类卵巢排卵前卵泡的细胞中，可能是卵泡在黄体化过程中的正常生理过程。
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