Birren B W, Hood L, Lai E
Division of Biology, California Institute of Technology, Pasadena 91125.
Electrophoresis. 1989 May-Jun;10(5-6):302-9. doi: 10.1002/elps.1150100506.
We have studied the migration of DNA in pulsed field agarose gels under a variety of electrophoresis conditions. We have made use of an instrument which can generate electric fields of any orientation, magnitude, or duration to compare different separation techniques for DNA molecules of from 1 to several thousand kilobase pairs. We discuss the capabilities of the system and present results of gel runs in which electrophoresis conditions were changed individually or in combination. The mobility of DNA in pulsed field gels is shown to reflect a number of interdependent physical parameters.
我们研究了在各种电泳条件下DNA在脉冲场琼脂糖凝胶中的迁移情况。我们使用了一种能够产生任意方向、大小或持续时间电场的仪器，以比较用于分离1至数千千碱基对DNA分子的不同技术。我们讨论了该系统的性能，并展示了在单独或组合改变电泳条件的凝胶电泳实验结果。结果表明，DNA在脉冲场凝胶中的迁移率反映了许多相互依存的物理参数。
