Korosec T, Gerner C, Sauermann G
Institute of Tumor Biology-Cancer Research, University of Vienna, Austria.
Electrophoresis. 1997 Oct;18(11):2109-15. doi: 10.1002/elps.1150181138.
Nuclear matrix proteins have been defined as insoluble residual proteins resulting from treatment of isolated nuclei with nucleases, detergents and high ionic strength buffers. They are considered as in part representing the proteins constituting the three-dimensional framework of the interphase nucleus. Though cell-specific nuclear matrix proteins have been differentiated from ubiquitously occurring (common) nuclear matrix proteins, the number and types of common nuclear matrix proteins have not yet been unequivocally established. In the present study nuclear matrix proteins were prepared from isolated nuclei of rat kidney, liver, lung, spleen and testes. The matrix proteins were separated by two-dimensional (2-D) electrophoresis and silver stained. Then the spot patterns were compared by computer-assisted image analysis. Composite images were derived for nuclear matrix proteins of individual tissues. Finding between 396-483 spots per tissue, a total of 964 individual spots were registered. Of these, 102 were common nuclear matrix proteins, as appearing in each of the tissue-characteristic images. The apparent molecular mass and pI data may serve for further identification of these nuclear proteins.
核基质蛋白被定义为用核酸酶、去污剂和高离子强度缓冲液处理分离的细胞核后产生的不溶性残余蛋白。它们被认为部分代表构成间期细胞核三维框架的蛋白质。尽管细胞特异性核基质蛋白已与普遍存在的（常见的）核基质蛋白区分开来，但常见核基质蛋白的数量和类型尚未明确确定。在本研究中，从大鼠肾脏、肝脏、肺、脾脏和睾丸的分离细胞核中制备核基质蛋白。通过二维（2-D）电泳分离基质蛋白并进行银染。然后通过计算机辅助图像分析比较斑点模式。得出了各个组织的核基质蛋白的合成图像。每个组织发现396 - 483个斑点，总共记录了964个单独的斑点。其中，102个是常见核基质蛋白，出现在每个组织特征图像中。表观分子量和pI数据可用于进一步鉴定这些核蛋白。
