病毒启动子/增强子元件与异源调控序列的交换产生用于黑色素瘤基因治疗的靶向杂交长末端重复序列载体。
Exchange of viral promoter/enhancer elements with heterologous regulatory sequences generates targeted hybrid long terminal repeat vectors for gene therapy of melanoma.
作者信息
Diaz R M, Eisen T, Hart I R, Vile R G
机构信息
Richard Dimbleby Department of Cancer Research/ICRF Laboratory, Rayne Institute, St. Thomas' Hospital, London, United Kingdom.
出版信息
J Virol. 1998 Jan;72(1):789-95. doi: 10.1128/JVI.72.1.789-795.1998.
Abstract
To generate transcriptionally targeted vectors, tissue-specific elements of the human tyrosinase promoter were exchanged with corresponding viral elements in the Moloney murine leukemia virus long terminal repeat (LTR). From these experiments, a vesicular stomatitis virus type G pseudotyped, hybrid LTR vector that contained three tyrosinase enhancer elements and gave high-level, tightly tissue-specific expression at high titers (3 x 10(7) CFU/ml) was constructed.
摘要
为了构建转录靶向载体，将人酪氨酸酶启动子的组织特异性元件与莫洛尼鼠白血病病毒长末端重复序列（LTR）中的相应病毒元件进行了交换。通过这些实验，构建了一种水泡性口炎病毒G型假型化的杂交LTR载体，该载体包含三个酪氨酸酶增强子元件，能够在高滴度（3×10⁷CFU/ml）下实现高水平、严格组织特异性的表达。
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