Caruso A, Licenziati S, Canaris A D, Cantalamessa A, Fiorentini S, Ausenda S, Ricotta D, Dima F, Malacarne F, Balsari A, Turano A
Institute of Microbiology, University of Brescia Medical School, Spedali Civili, 25123 Brescia, Italy.
J Clin Invest. 1998 Jan 1;101(1):137-44. doi: 10.1172/JCI195.
The relationship between the number of circulating CD4+ T cells and the presence of particular CD8+ T cell subsets was analyzed by flow cytometry on PBL from asymptomatic HIV-1-infected patients whose specimens were collected every 2 mo for a total period of 32 mo. Only slight variations were detected in the absolute number of lymphocytes and percentage of CD3+ lymphocytes, whereas both CD4+ and CD8+ T cell subsets showed wide intrapatient variation. Variations in the number of CD8+CD28+ cells paralleled those of the CD4+ T cell subset in each patient tested, while the presence of CD8+CD28- T cells correlated inversely with CD4+ and CD8+CD28+ T cells. These data show that changes in the number of circulating CD4+-and CD8+CD28+ T cells are strongly related to the presence of CD8+CD28- T cells in these patients. Insight into the significance of CD8+CD28- T cell expansion will allow us to understand the mechanisms and significance of the HIV-1- driven change in CD4+CD8+ T cell homeostasis and the basic immunopathology of HIV disease.
通过流式细胞术对无症状HIV-1感染患者的外周血淋巴细胞(PBL)进行分析,这些患者的标本每2个月采集一次,共采集32个月,以分析循环CD4 + T细胞数量与特定CD8 + T细胞亚群存在之间的关系。淋巴细胞的绝对数量和CD3 +淋巴细胞的百分比仅检测到轻微变化,而CD4 +和CD8 + T细胞亚群在患者体内均表现出广泛的变化。在每个测试患者中,CD8 + CD28 +细胞数量的变化与CD4 + T细胞亚群的变化平行,而CD8 + CD28- T细胞的存在与CD4 +和CD8 + CD28 + T细胞呈负相关。这些数据表明,在这些患者中,循环CD4 +和CD8 + CD28 + T细胞数量的变化与CD8 + CD28- T细胞的存在密切相关。深入了解CD8 + CD28- T细胞扩增的意义将使我们能够理解HIV-1驱动的CD4 + CD8 + T细胞稳态变化的机制和意义以及HIV疾病的基本免疫病理学。