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猪带绦虫虫卵孵化方法及六钩蚴活力测定的比较研究

A comparative study on egg hatching methods and oncosphere viability determination for Taenia solium eggs.

作者信息

Wang I C, Ma Y X, Kuo C H, Fan P C

机构信息

Institute and Department of Parasitology, National Yangming University, Taipei, Taiwan, People's Republic of China.

出版信息

Int J Parasitol. 1997 Nov;27(11):1311-4. doi: 10.1016/s0020-7519(97)00087-8.

Abstract

The efficiencies of an enzyme method and the sodium hypochlorite method in hatching eggs of Taenia solium (Henan strain) were compared. The viability of the hatched oncospheres was determined by staining with trypan blue. For the former method, the hatching rate and viability percentage were 85.7% and 79.7%, respectively. The corresponding figures for the latter method were 97.9% and 86.7%, respectively. These findings indicate that the sodium hypochlorite method is a simple, efficient and practical method for hatching eggs of Taenia solium.

摘要

比较了酶法和次氯酸钠法对猪带绦虫(河南株)虫卵的孵化效率。用台盼蓝染色法测定孵出的六钩蚴的活力。对于前一种方法,孵化率和活力百分比分别为85.7%和79.7%。后一种方法的相应数字分别为97.9%和86.7%。这些结果表明,次氯酸钠法是一种简单、高效且实用的猪带绦虫虫卵孵化方法。

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