从家蝇的胃肠道和外骨骼中回收猪带绦虫和猪蛔虫卵的方案的性能评估。

Performance evaluation of protocols for Taenia saginata and Ascaris suum egg recovery from the house fly's gastrointestinal tract and exoskeleton.

机构信息

Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.

Biometrics Research Group, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.

出版信息

Parasit Vectors. 2023 Dec 18;16(1):459. doi: 10.1186/s13071-023-06077-5.

DOI:10.1186/s13071-023-06077-5

PMID:38110985

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10729472/

Abstract

BACKGROUND

The synanthropic house fly (Musca domestica) can potentially contribute to the mechanical spread of eggs of Taenia and Ascaris spp. in the environment and between hosts. However, the absence of validated protocols to recover eggs hampers an in-depth analysis of the house fly's role in parasite egg transmission.

METHODS

The gastrointestinal tract and exoskeleton of euthanized house flies were spiked with Taenia saginata eggs. The performance of several recovery protocols, in terms of both the recovery rate and ease-of-use, was (microscopically) evaluated and compared. These protocols employed steps such as washing, maceration, filtration, flotation and both passive and centrifugal sedimentation. The final validated protocols were subsequently evaluated for the recovery of Ascaris suum eggs.

RESULTS

The final protocol validated for the recovery of T. saginata eggs from the house fly's gastrointestinal tract involved homogenization in phosphate-buffered saline and centrifugation at 2000 g for 2 min, yielding a recovery rate of 79.7%. This protocol required 6.5 min to perform (which included 1.5 min of hands-on time) and removed large debris particles that could hinder the differentiation of eggs from debris. Similarly, the final protocol validated for the recovery of T. saginata eggs from the fly's exoskeleton involved washing by vortexing for 2 min in Tween 80 (0.05%), 15 min of passive sedimentation and centrifugation at 2000 g for 2 min, yielding a recovery rate of 77.4%. This protocol required 20.5 min to perform (which included 3.5 min of hands-on time) and successfully removed debris. The same protocols yielded recovery rates of 74.2% and 91.5% for the recovery of A. suum eggs from the fly's gastrointestinal tract and exoskeleton, respectively.

CONCLUSIONS

Effective, simple and easy-to-use protocols were developed and validated for the recovery of T. saginata and A. suum eggs from the house fly's gastrointestinal tract and exoskeleton. These protocols can be applied to investigate the importance of flies as parasite egg transmitters in laboratory and field settings.

摘要

背景

与人共生的家蝇（Musca domestica）可能有助于在环境中和宿主之间传播绦虫和蛔虫属的卵。然而，由于缺乏验证的卵回收方案，阻碍了对家蝇在寄生虫卵传播中的作用进行深入分析。

方法

用牛带绦虫卵感染安乐死的家蝇的胃肠道和外骨骼。从回收率和易用性两方面评估并比较了几种回收方案的性能。这些方案采用了洗涤、捣碎、过滤、浮选以及被动和离心沉淀等步骤。最后验证的方案随后用于回收猪蛔虫卵。

结果

从家蝇胃肠道中回收牛带绦虫卵的最终验证方案涉及磷酸盐缓冲盐水匀浆和 2000g 离心 2 分钟，回收率为 79.7%。该方案的执行时间为 6.5 分钟（其中包括 1.5 分钟的实际操作时间），并去除了可能阻碍卵与碎片区分的大碎片颗粒。同样，从蝇外骨骼中回收牛带绦虫卵的最终验证方案涉及在吐温 80（0.05%）中涡旋洗涤 2 分钟、15 分钟的被动沉淀和 2000g 离心 2 分钟，回收率为 77.4%。该方案的执行时间为 20.5 分钟（其中包括 3.5 分钟的实际操作时间），并成功去除了碎片。同样的方案分别从家蝇的胃肠道和外骨骼中回收猪蛔虫卵的回收率为 74.2%和 91.5%。