对一段5.5千碱基对的DNA片段进行测序，并鉴定编码禽喉气管炎病毒（ILTV）解旋酶/引发酶复合体一个亚基的基因。

Sequencing of a 5.5-kb DNA fragment and identification of a gene coding for a subunit of the helicase/primase complex of avian laryngotracheitis virus (ILTV).

作者信息

Huang Q, Mat-Arip Y, Guo P

机构信息

Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, USA.

出版信息

Virus Genes. 1997;15(2):119-21. doi: 10.1023/a:1007958807339.

DOI:10.1023/a:1007958807339

PMID:9421876

Abstract

The nucleotide sequence of a 5,520-bp EcoRI restriction fragment of avian infectious laryngotracheitis (ILTV) DNA was reported and submitted to GeneBank with an accession number of AF001078. Computer prediction revealed one large potential open reading frame (ORF) with sequence similar to one subunit of the DNA helicase-primase complex of alpha-herpesviruses. The DNA helicase/primase complex of HSV-1 consists of three sub-units with molecular weights of 12,000, 97,000 and 70,000, encoded by genes UL52, UL5 and UL8, respectively. This enzyme complex is essential for herpesvirus DNA replication. The UL52 and UL5-equivalent genes of ILTV have been reported previously (Fuchs, W. and Mettenleiter, T.C.; J Gen Virol, 1996, 77: 2221-2229; Johnson, M.A. et. al., Arch Virol, 1995, 14: 623-634). Amino acid sequence comparison and homology search revealed that this ORF shares sequence similarity of the UL8-equivalent gene of alpha-herpesviruses, that is, the ORF 52 of vericura-zoster virus (VZV), the ORF 54 of equine herpesvirus type-1 (EHV-1), as well as the equivalent gene of bovine herpesvirus type 1 (BHV-1) and canine herpesvirus (Vlcek, C. et al., Virology, 1995, 210: 100-108; Remond, M. et al., J Gen Virol, 1996, 77: 37-48).

摘要

报道了禽传染性喉气管炎病毒（ILTV）DNA的一个5520碱基对的EcoRI限制性片段的核苷酸序列，并提交至基因库，登录号为AF001078。计算机预测显示有一个大的潜在开放阅读框（ORF），其序列与α-疱疹病毒的DNA解旋酶-引发酶复合体的一个亚基相似。单纯疱疹病毒1型（HSV-1）的DNA解旋酶/引发酶复合体由三个亚基组成，分子量分别为12000、97000和70000，分别由基因UL52、UL5和UL8编码。这种酶复合体对疱疹病毒DNA复制至关重要。ILTV的UL52和UL5等同基因此前已有报道（Fuchs, W.和Mettenleiter, T.C.；《普通病毒学杂志》，1996年，77卷：2221 - 2229页；Johnson, M.A.等人，《病毒学文献》，1995年，14卷：623 - 634页）。氨基酸序列比较和同源性搜索显示，这个ORF与α-疱疹病毒的UL8等同基因具有序列相似性，即水痘-带状疱疹病毒（VZV）的ORF 52、马疱疹病毒1型（EHV-1）的ORF 54，以及牛疱疹病毒1型（BHV-1）和犬疱疹病毒的等同基因（Vlcek, C.等人，《病毒学》，1995年，210卷：100 - 108页；Remond, M.等人，《普通病毒学杂志》，1996年，77卷：37 - 48页）。