Kingsley D H, Hazel J W, Keeler C L
Department of Animal Science and Agricultural Biochemistry, Delaware Agricultural Experiment Station, College of Agricultural Sciences, University of Delaware, Newark 19717-1303.
Virology. 1994 Sep;203(2):336-43. doi: 10.1006/viro.1994.1492.
The infectious laryngotracheitis virus (ILTV) gene encoding a homologue to the glycoprotein C gene of herpes simplex virus has been sequenced and identified based on its genomic location, comparative analysis to other gC proteins, and the identification of a glycosylated protein product. Located near the small subunit ribonucleotide reductase gene, the ILTV gC gene is 1242 bp in length and is predicted to encode a membrane glycoprotein containing a characteristic N-terminal hydrophobic signal sequence, five potential N-linked glycosylation sites, and C-terminal transmembrane and cytoplasmic domains. Antibodies raised in rabbits against a Cro-ILTV-beta-galactosidase fusion protein expressed in Escherichia coli recognize a 60-kDa ILTV-specific glycoprotein from infected cell extracts. Transcriptional analysis, using a portion of the open reading frame as a probe, identified a 1.55-kb transcript expressed with late gene kinetics. Comparison to other herpesvirus gC proteins revealed limited amino acid sequence homology and the absence of a charged extracellular region, which would normally interact with cell surface proteoglycans.
编码与单纯疱疹病毒糖蛋白C基因同源物的传染性喉气管炎病毒（ILTV）基因已根据其基因组位置、与其他gC蛋白的比较分析以及糖基化蛋白产物的鉴定进行了测序和鉴定。ILTV gC基因位于小亚基核糖核苷酸还原酶基因附近，长度为1242 bp，预计编码一种膜糖蛋白，该蛋白含有特征性的N端疏水信号序列、五个潜在的N-连接糖基化位点以及C端跨膜和细胞质结构域。用在大肠杆菌中表达的Cro-ILTV-β-半乳糖苷酶融合蛋白免疫家兔产生的抗体可识别感染细胞提取物中一种60 kDa的ILTV特异性糖蛋白。使用开放阅读框的一部分作为探针进行转录分析，鉴定出一个以晚期基因动力学表达的1.55 kb转录本。与其他疱疹病毒gC蛋白的比较显示氨基酸序列同源性有限，并且没有通常会与细胞表面蛋白聚糖相互作用的带电荷细胞外区域。
