Schwartz R D, McCoy C J
Appl Environ Microbiol. 1976 Jan;31(1):78-82. doi: 10.1128/aem.31.1.78-82.1976.
The kinetics of the enzymatic formation of 7,8-epoxy-1-octene, 1,2-7,8-diepoxyoctane, and 1,2-epoxyoctane by growing and resting cell suspensions of Pseudomonas oleovorans are described. Formation of 1,2-epoxyoctane occurs concurrently with exponential growth on 1-octene, providing that 1-octene is in excess. Conversion of 1,7-octadiene to 7,8-epoxy-1-octene by cells growing on octane lags behind exponential growth and continues into the stationary phase, terminating upon cell death. Formation of 1,2-7,8-diepoxyoctane does not begin until the cells are well into the stationary phase and also continues until cell death. Results with growing and resting cell suspensions suggest that the various substrates compete for the same enzyme system; that viable cells are essential for substrate transport and epoxidation by whole cells; and that whole cells may concentrate and sequester the epoxides, rendering them unrecoverable by our current methods.
描述了食油假单胞菌生长细胞和静止细胞悬浮液将1-辛烯酶促形成7,8-环氧-1-辛烯、1,2-7,8-二环氧辛烷和1,2-环氧辛烷的动力学。只要1-辛烯过量，1,2-环氧辛烷的形成与在1-辛烯上的指数生长同时发生。在辛烷上生长的细胞将1,7-辛二烯转化为7,8-环氧-1-辛烯的过程滞后于指数生长，并持续到稳定期，在细胞死亡时终止。1,2-7,8-二环氧辛烷的形成直到细胞进入稳定期才开始，并且也持续到细胞死亡。生长细胞和静止细胞悬浮液的结果表明，各种底物竞争同一酶系统；活细胞对于底物运输和全细胞环氧化至关重要；并且全细胞可能会浓缩和隔离环氧化物，使它们无法通过我们目前的方法回收。
