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骨髓移植后血型测定的方法依赖性

Method dependency of blood group determination after bone marrow transplantation.

作者信息

Wagner F, Wölpl A, Greher J, Kubanek B

机构信息

Abteilung Transfusionsmedizin, Universität Ulm, Germany.

出版信息

Beitr Infusionsther Transfusionsmed. 1994;32:171-4.

PMID:9422110
Abstract

After minor-incompatible bone marrow transplantation (e.g. recipient A, donor 0), red cells are donor-type but absorb recipient-type AB0 substance from plasma. Because of discrepant results, we tested how such cells type with standard methods. In the tube test 7 of 8 patients showed donor type. In Diamed AB0 gel, weak-positive reactions were found with anti-AB in the patients (donor 0) tested. Commercial AB0 antibodies from different companies used in Diamed neutral gel trays differed widely in the results obtained. The strongest positive reactions were found with polyclonal anti-AB. The exact antibody and technique are crucial to the result of AB0 typing after minor-incompatible bone marrow transplantation.

摘要

在进行次要不相容的骨髓移植后(例如受者为A血型,供者为O血型),红细胞为供者型,但会从血浆中吸收受者型ABO物质。由于结果存在差异,我们测试了此类细胞用标准方法进行血型鉴定的情况。在试管试验中,8名患者中有7名显示为供者血型。在达美ABO凝胶试验中,对所检测的患者(供者为O型)用抗AB检测发现弱阳性反应。在达美中性凝胶板中使用不同公司的商用ABO抗体,所获得的结果差异很大。用多克隆抗AB检测时阳性反应最强。准确的抗体和技术对于次要不相容骨髓移植后ABO血型鉴定的结果至关重要。

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