Fregatova L, Volkova O, Platonova G, Afanasiev B
Petrov Research Institute of Oncology, Clinical Center of Advanced Medical Technologies, St.-Petersburg, Russia.
Beitr Infusionsther Transfusionsmed. 1994;32:445-7.
Incompatibility of blood group antigens in bone marrow transplantation (BMT) requires the depletion of red blood cells from donor bone marrow in order to avoid fatal transfusion reactions. We purified 72 human bone marrows prior to transplantation from red blood cells by a modified method using simple centrifugation and sedimentation in 6% dextran. Small-volume samples (n = 4) were prediluted with compatible homologous red blood cells to reduce the loss of stem cells during the preparation steps. 12 of 30 allogeneic bone marrows were ABO major incompatible with the patient's blood group. Although we did not observe any transfusion reactions nor graft rejections, in ABO incompatible transplantations, donor reticulocytes appeared 10 days later in the peripheral blood than in patients who received an ABO compatible bone marrow. Concordantly, incompatible patients needed 1.7 times more red blood cell units after BMT.
骨髓移植（BMT）中血型抗原的不相容性要求去除供体骨髓中的红细胞，以避免致命的输血反应。我们采用一种改良方法，通过在6%葡聚糖中进行简单离心和沉降，在移植前从72份人骨髓中纯化出红细胞。小体积样本（n = 4）用相容的同源红细胞进行预稀释，以减少制备步骤中干细胞的损失。30份同种异体骨髓中有12份与患者血型存在ABO主要不相容。尽管我们未观察到任何输血反应或移植物排斥反应，但在ABO不相容的移植中，供体网织红细胞在外周血中出现的时间比接受ABO相容骨髓的患者晚10天。相应地，不相容患者在骨髓移植后需要的红细胞单位数量是前者的1.7倍。
