Chan W Y, Wong N, Chan A B, Chow J H, Lee J C
Department of Anatomical & Cellular Pathology, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Am J Pathol. 1998 Jan;152(1):11-6.
Fifteen cases of high grade primary gastric non-Hodgkin's lymphomas were studied using comparative genomic hybridization (CGH) and/or fluorescence in situ hybridization (FISH) techniques. A total of 10 cases of diffuse large cell lymphoma (DLCL) with no histologically identifiable or previous history of low grade mucosa-associated lymphoid tissue (MALT) lymphoma components were examined, four by CGH and validated by FISH, and the remaining six by FISH alone. All 10 tumors showed gains in chromosome 12. Other recurring CGH findings in DLCL included copy number gains of 1q and deletions of 6q. Five cases of high grade tumors with low grade MALT components (HGM) were also examined, three by CGH and validated by FISH and two by FISH only. Only one in five HGM showed gains of chromosome 12. Other recurring CGH findings in HGM included +7q and +11q. We conclude that high grade gastric lymphomas of DLCL type were associated with gains in chromosome 12. The change was much less frequent (P < 0.01) in the HGM type, which had a percentage similar to that observed in previously reported cytogenetics/FISH studies on low grade MALT lymphomas. Our findings suggested that many DLCL were not derived from transformation of low grade MALT lymphomas.
采用比较基因组杂交（CGH）和/或荧光原位杂交（FISH）技术对15例原发性高级别胃非霍奇金淋巴瘤进行了研究。共检查了10例无组织学可识别的或既往低级别黏膜相关淋巴组织（MALT）淋巴瘤成分病史的弥漫性大细胞淋巴瘤（DLCL），其中4例采用CGH检查并经FISH验证，其余6例仅采用FISH检查。所有10例肿瘤均显示12号染色体获得。DLCL中其他反复出现的CGH结果包括1q拷贝数增加和6q缺失。还检查了5例伴有低级别MALT成分的高级别肿瘤（HGM），其中3例采用CGH检查并经FISH验证，2例仅采用FISH检查。5例HGM中只有1例显示12号染色体获得。HGM中其他反复出现的CGH结果包括7q增加和11q增加。我们得出结论，DLCL型高级别胃淋巴瘤与12号染色体获得相关。在HGM型中这种改变的频率要低得多（P<0.01），其百分比与先前报道的关于低级别MALT淋巴瘤的细胞遗传学/FISH研究中观察到的相似。我们的研究结果表明，许多DLCL并非由低级别MALT淋巴瘤转化而来。
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