二酰基甘油激酶参与变形链球菌羊毛硫抗生素变链菌素II表达的调控。
Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans.
作者信息
Chen P, Novak J, Qi F, Caufield P W
机构信息
Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, 35294, USA.
出版信息
J Bacteriol. 1998 Jan;180(1):167-70. doi: 10.1128/JB.180.1.167-170.1998.
Abstract
Genetic characterization of a Tn916 transposon mutant, Streptococcus mutans T8-1, defective in mutacin II production, revealed that the transposon was inserted into the 3' region of a diacylglycerol kinase (dgk) gene. The insertion occurred in the same region as described for another S. mutans mutant, GS5Tn1, which was altered in its ability to respond to environmental stress (Y. Yamashita, T. Takehara, and H. K. Kuramitsu, J. Bacteriol. 175:6220-6228, 1993). Quantitative primer extension from the mutacin structural gene mutA showed a decreased level (about eightfold) of mutA transcription for mutant T8-1. Mutacin production was restored by transforming mutant T8-1 with integration vector pVA891 containing an intact dgk gene. These data indicated that the full-length dgk gene product along with the mutacin biosynthetic operon are required for the production of the mutacin II lantibiotic.
摘要
对变形链球菌T8-1（一种在变链菌素II产生方面存在缺陷的Tn916转座子突变体）的遗传特征分析表明，转座子插入到了二酰基甘油激酶（dgk）基因的3'区域。该插入发生在与另一个变形链球菌突变体GS5Tn1所述相同的区域，GS5Tn1在应对环境压力的能力方面发生了改变（Y. 山下、T. 竹原和H. K. 仓光，《细菌学杂志》175:6220 - 6228, 1993）。从变链菌素结构基因mutA进行的定量引物延伸显示，突变体T8-1的mutA转录水平降低（约八倍）。通过用含有完整dgk基因的整合载体pVA891转化突变体T8-1，变链菌素的产生得以恢复。这些数据表明，全长dgk基因产物以及变链菌素生物合成操纵子是产生变链菌素II羊毛硫抗生素所必需的。
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