变形链球菌T8中羊毛硫抗生素变链菌素II生物合成的特定基因成簇存在，且可作为一个整体进行转移。
The specific genes for lantibiotic mutacin II biosynthesis in Streptococcus mutans T8 are clustered and can be transferred en bloc.
作者信息
Chen P, Qi F, Novak J, Caufield P W
机构信息
Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
出版信息
Appl Environ Microbiol. 1999 Mar;65(3):1356-60. doi: 10.1128/AEM.65.3.1356-1360.1999.
Abstract
Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans. DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutR), the prepromutacin structural gene (mutA), a modifying protein (mutM), an ABC transporter (mutT), and an immunity cluster (mutFEG). Transformations of a non-mutacin-producing strain, S. mutans UA159, and a mutacin I-producing strain, S. mutans UA140, with chromosomal DNA from S. mutans T8 with an aphIII marker inserted upstream of the mutacin II structural gene yielded transformants producing mutacin II and mutacins I and II, respectively.
摘要
变链菌素II是由II组变形链球菌产生的一种核糖体合成的肽类羊毛硫抗生素。DNA测序表明，变链菌素II生物合成基因簇由七个特定的开放阅读框组成：一个调节基因（mutR）、前变链菌素结构基因（mutA）、一个修饰蛋白（mutM）、一个ABC转运蛋白（mutT）和一个免疫簇（mutFEG）。用来自变形链球菌T8的染色体DNA（在变链菌素II结构基因上游插入了aphIII标记）转化非产变链菌素菌株变形链球菌UA159和产变链菌素I菌株变形链球菌UA140，分别产生了产变链菌素II以及产变链菌素I和II的转化体。
相似文献
1
The specific genes for lantibiotic mutacin II biosynthesis in Streptococcus mutans T8 are clustered and can be transferred en bloc.变形链球菌T8中羊毛硫抗生素变链菌素II生物合成的特定基因成簇存在，且可作为一个整体进行转移。
Appl Environ Microbiol. 1999 Mar;65(3):1356-60. doi: 10.1128/AEM.65.3.1356-1360.1999.
2
Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutacin III biosynthesis genes.从变形链球菌III群UA787中纯化变链菌素III并对变链菌素III生物合成基因进行遗传分析。
Appl Environ Microbiol. 1999 Sep;65(9):3880-7. doi: 10.1128/AEM.65.9.3880-3887.1999.
3
The group I strain of Streptococcus mutans, UA140, produces both the lantibiotic mutacin I and a nonlantibiotic bacteriocin, mutacin IV.变形链球菌I群菌株UA140可产生羊毛硫抗生素变链菌素I和一种非羊毛硫抗生素细菌素——变链菌素IV。
Appl Environ Microbiol. 2001 Jan;67(1):15-21. doi: 10.1128/AEM.67.1.15-21.2001.
4
Sequence analysis of mutA and mutM genes involved in the biosynthesis of the lantibiotic mutacin II in Streptococcus mutans.变形链球菌中参与羊毛硫抗生素变链菌素II生物合成的mutA和mutM基因的序列分析。
Gene. 1998 Jan 5;206(1):37-43. doi: 10.1016/s0378-1119(97)00578-7.
5
Functional analyses of the promoters in the lantibiotic mutacin II biosynthetic locus in Streptococcus mutans.变形链球菌中羊毛硫抗生素变链菌素II生物合成基因座中启动子的功能分析。
Appl Environ Microbiol. 1999 Feb;65(2):652-8. doi: 10.1128/AEM.65.2.652-658.1999.
6
Isolation and partial characterization of the Streptococcus mutans type AII lantibiotic mutacin K8.变形链球菌AII型羊毛硫抗生素变链菌素K8的分离及部分特性分析
Microbiology (Reading). 2007 May;153(Pt 5):1631-1641. doi: 10.1099/mic.0.2006/003756-0.
7
Effect of amino acid substitutions in conserved residues in the leader peptide on biosynthesis of the lantibiotic mutacin II.前导肽中保守残基的氨基酸替换对羊毛硫抗生素变链菌素II生物合成的影响。
FEMS Microbiol Lett. 2001 Feb 20;195(2):139-44. doi: 10.1111/j.1574-6968.2001.tb10511.x.
8
Genetic Analysis of Mutacin B-Ny266, a Lantibiotic Active against Caries Pathogens.Mutacin B-Ny266 的遗传分析，一种针对龋齿病原菌的类细菌素。
J Bacteriol. 2020 May 27;202(12). doi: 10.1128/JB.00762-19.
9
Structure-activity study of the lantibiotic mutacin II from Streptococcus mutans T8 by a gene replacement strategy.通过基因替换策略对变形链球菌T8的羊毛硫抗生素变链菌素II进行结构-活性研究。
Appl Environ Microbiol. 1998 Jul;64(7):2335-40. doi: 10.1128/AEM.64.7.2335-2340.1998.
10
Gene Rearrangement and Modification of Immunity Factors Are Correlated with the Insertion of Bacteriocin Cassettes in Streptococcus mutans.基因重排和免疫因子修饰与细菌素盒在变形链球菌中的插入有关。
Microbiol Spectr. 2022 Jun 29;10(3):e0180621. doi: 10.1128/spectrum.01806-21. Epub 2022 May 23.
引用本文的文献
1
Synthesis of a Novel Lantibiotic Using Mutacin II Biosynthesis Apparatus.利用Mutacin II 生物合成装置合成新型类菌素。
Microbiol Spectr. 2023 Feb 14;11(1):e0303022. doi: 10.1128/spectrum.03030-22. Epub 2023 Jan 16.
2
Proteomic Characterization and Target Identification Against Streptococcus mutans Under Bacitracin Stress Conditions Using LC-MS and Subtractive Proteomics.使用 LC-MS 和消减蛋白质组学技术对抗生素杆菌素胁迫条件下变异链球菌的蛋白质组学特征分析和靶标鉴定
Protein J. 2022 Feb;41(1):166-178. doi: 10.1007/s10930-021-10038-1. Epub 2022 Jan 6.
3
Quorum Sensing in Streptococcus mutans Regulates Production of Tryglysin, a Novel RaS-RiPP Antimicrobial Compound.变形链球菌群体感应调控胰酶素（一种新型 Ras-RiPP 类抗菌化合物）的产生。
mBio. 2021 Mar 16;12(2):e02688-20. doi: 10.1128/mBio.02688-20.
4
A single system detects and protects the beneficial oral bacterium Streptococcus sp. A12 from a spectrum of antimicrobial peptides.一种单一系统可检测并保护有益口腔细菌链球菌 sp. A12 免受一系列抗菌肽的影响。
Mol Microbiol. 2021 Jul;116(1):211-230. doi: 10.1111/mmi.14703. Epub 2021 Feb 25.
5
Genome-Guided Mass Spectrometry Expedited the Discovery of Paraplantaricin TC318, a Lantibiotic Produced by Strain Isolated From Cheese.基因组引导的质谱分析加速了副植物乳杆菌素TC318的发现，TC318是一种从奶酪中分离出的菌株产生的羊毛硫抗生素。
Front Microbiol. 2020 Jul 14;11:1381. doi: 10.3389/fmicb.2020.01381. eCollection 2020.
6
Complete Genome Sequences of Two Mutacin-Producing Streptococcus mutans Strains, T8 and UA140.两株产变链菌素的变形链球菌菌株T8和UA140的全基因组序列
Microbiol Resour Announc. 2020 Jun 11;9(24):e00469-20. doi: 10.1128/MRA.00469-20.
7
Comparing the cariogenic species Streptococcus sobrinus and S. mutans on whole genome level.在全基因组水平上比较致龋菌远缘链球菌和变形链球菌。
J Oral Microbiol. 2014 Dec 3;6:26189. doi: 10.3402/jom.v6.26189. eCollection 2014.
8
Antimicrobial Peptide Resistance Mechanisms of Gram-Positive Bacteria.革兰氏阳性菌的抗菌肽耐药机制。
Antibiotics (Basel). 2014 Oct 13;3(4):461-92. doi: 10.3390/antibiotics3040461.
9
Genetic variability of mutans streptococci revealed by wide whole-genome sequencing.宽全基因组测序揭示变形链球菌的遗传变异性。
BMC Genomics. 2013 Jun 28;14:430. doi: 10.1186/1471-2164-14-430.
10
The mutacins of Streptococcus mutans: regulation and ecology.变形链球菌素的调节与生态。
Mol Oral Microbiol. 2012 Apr;27(2):57-69. doi: 10.1111/j.2041-1014.2011.00634.x. Epub 2011 Dec 23.
本文引用的文献
1
Structure-activity study of the lantibiotic mutacin II from Streptococcus mutans T8 by a gene replacement strategy.通过基因替换策略对变形链球菌T8的羊毛硫抗生素变链菌素II进行结构-活性研究。
Appl Environ Microbiol. 1998 Jul;64(7):2335-40. doi: 10.1128/AEM.64.7.2335-2340.1998.
2
Sequence analysis of mutA and mutM genes involved in the biosynthesis of the lantibiotic mutacin II in Streptococcus mutans.变形链球菌中参与羊毛硫抗生素变链菌素II生物合成的mutA和mutM基因的序列分析。
Gene. 1998 Jan 5;206(1):37-43. doi: 10.1016/s0378-1119(97)00578-7.
3
Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans.二酰基甘油激酶参与变形链球菌羊毛硫抗生素变链菌素II表达的调控。
J Bacteriol. 1998 Jan;180(1):167-70. doi: 10.1128/JB.180.1.167-170.1998.
4
Characterization of the lacticin 481 operon: the Lactococcus lactis genes lctF, lctE, and lctG encode a putative ABC transporter involved in bacteriocin immunity.乳酸乳球菌素481操纵子的特性：乳酸乳球菌基因lctF、lctE和lctG编码一种参与细菌素免疫的推定ABC转运蛋白。
Appl Environ Microbiol. 1997 Nov;63(11):4252-60. doi: 10.1128/aem.63.11.4252-4260.1997.
5
Molecular characterization of the plasmid-encoded eps gene cluster essential for exopolysaccharide biosynthesis in Lactococcus lactis.乳酸乳球菌中胞外多糖生物合成所必需的质粒编码eps基因簇的分子特征
Mol Microbiol. 1997 Apr;24(2):387-97. doi: 10.1046/j.1365-2958.1997.3521720.x.
6
Definition of the single integration site of the pathogenicity locus in Clostridium difficile.艰难梭菌致病位点单一整合位点的定义。
Gene. 1996 Nov 28;181(1-2):29-38. doi: 10.1016/s0378-1119(96)00398-8.
7
Rgg is a positive transcriptional regulator of the Streptococcus gordonii gtfG gene.Rgg是戈登链球菌gtfG基因的一种正向转录调节因子。
J Bacteriol. 1996 Oct;178(19):5826-30. doi: 10.1128/jb.178.19.5826-5830.1996.
8
Post-translational modifications of lantibiotics.羊毛硫抗生素的翻译后修饰。
Antonie Van Leeuwenhoek. 1996 Feb;69(2):139-50. doi: 10.1007/BF00399419.
9
Novel lantibiotics and their pre-peptides.新型羊毛硫抗生素及其前肽。
Antonie Van Leeuwenhoek. 1996 Feb;69(2):89-97. doi: 10.1007/BF00399414.
10
The genetics of lantibiotic biosynthesis.羊毛硫抗生素生物合成的遗传学
Bioessays. 1995 Sep;17(9):793-802. doi: 10.1002/bies.950170909.
Zotero 插件