凝血酶原N端区域新氨基酸（γ-羧基谷氨酸）十个残基的质谱鉴定及序列定位
Mass-spectrometric identification and sequence location of the ten residues of the new amino acid (gamma-Carboxyglutamic acid) in the N-terminal region of prothrombin.
作者信息
Morris H R, Dell A
出版信息
Biochem J. 1976 Mar 1;153(3):663-79. doi: 10.1042/bj1530663.
Abstract
The detailed mass-spectrometric evidence for our original findings [Magnusson et al. (1974) FEBS Lett. 44, 189-193] of ten gamma-carboxyglutamic acid residues in the N-terminal calcium-binding polypeptide of prothrombin is presented. The identification and sequence location of gamma-carboxyglutamic acid was made by electron-impact and field-desorption studies on acetyl permethyl peptide derivatives, and on the free amino acid. Details of the derivatives formed, and how this new amino acid may be easily recognized and sequenced from the mass spectrum, are given as a basis for future work.
摘要
本文提供了详细的质谱分析证据，以证明我们最初的发现[Magnusson等人（1974年），《欧洲生物化学学会联合会快报》44卷，第189 - 193页]：凝血酶原N端钙结合多肽中存在十个γ-羧基谷氨酸残基。通过对乙酰基全甲基化肽衍生物和游离氨基酸进行电子轰击和场解吸研究，确定了γ-羧基谷氨酸的身份及其在序列中的位置。文中给出了所形成衍生物的详细信息，以及如何从质谱中轻松识别和测序这种新氨基酸，作为未来工作的基础。
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