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1
Mass-spectrometric identification and sequence location of the ten residues of the new amino acid (gamma-Carboxyglutamic acid) in the N-terminal region of prothrombin.凝血酶原N端区域新氨基酸（γ-羧基谷氨酸）十个残基的质谱鉴定及序列定位
Biochem J. 1976 Mar 1;153(3):663-79. doi: 10.1042/bj1530663.
2
Vitamin K dependent modifications of glutamic acid residues in prothrombin.凝血酶原中谷氨酸残基的维生素K依赖性修饰。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2730-3. doi: 10.1073/pnas.71.7.2730.
3
A mass-spectrometric method for the estimation of the ratio of gamma-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin.一种用于估算蛋白质特定位点上γ-羧基谷氨酸与谷氨酸比例的质谱方法。应用于牛凝血酶原的N端区域。
Biochem J. 1980 Apr 1;187(1):239-43. doi: 10.1042/bj1870239.
4
Vitamin K and the biosynthesis of prothrombin. V. Gamma-carboxyglutamic acids, the vitamin K-dependent structures in prothrombin.维生素K与凝血酶原的生物合成。V. γ-羧基谷氨酸，凝血酶原中维生素K依赖的结构。
J Biol Chem. 1975 Aug 10;250(15):6125-33.
5
The N-terminal sequences of blood coagulation factor X1 and X2 light chains. Mass-spectrometric identification of twelve residues of gamma-carboxyglutamic acid in their vitamin K-dependent domains.凝血因子X1和X2轻链的N端序列。其维生素K依赖结构域中12个γ-羧基谷氨酸残基的质谱鉴定。
Biochem J. 1978 Nov 1;175(2):613-27. doi: 10.1042/bj1750613.
6
Metal binding sites of a gamma-carboxyglutamic acid-rich fragment of bovine prothrombin.
J Biol Chem. 1979 Dec 25;254(24):12521-30.
7
Distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins.γ-羧基谷氨酸残基在部分羧化的人凝血酶原中的分布
J Biol Chem. 1986 Feb 5;261(4):1624-8.
8
Gamma-carboxyglutamic acid: identification and distribution in vitamin K-dependent proteins.γ-羧基谷氨酸：在维生素K依赖蛋白中的鉴定与分布
Mayo Clin Proc. 1974 Dec;49(12):941-4.
9
A method for specific chemical modification of gamma-carboxyglutamic acid residues in proteins.
Anal Biochem. 1984 May 15;139(1):82-90. doi: 10.1016/0003-2697(84)90392-0.
10
The mode of action of vitamin K. Identification of gamma-carboxyglutamic acid as a component of prothrombin.维生素K的作用方式。γ-羧基谷氨酸作为凝血酶原成分的鉴定。
J Biol Chem. 1974 Oct 10;249(19):6347-50.

引用本文的文献

1
Clinical application of protein induced by vitamin K antagonist-II as a biomarker in hepatocellular carcinoma.维生素K拮抗剂-II诱导蛋白作为生物标志物在肝细胞癌中的临床应用
Tumour Biol. 2016 Dec;37:15447–15456. doi: 10.1007/s13277-016-5443-x. Epub 2016 Oct 13.
2
A novel geometry mass spectrometer, the Q-TOF, for low-femtomole/attomole-range biopolymer sequencing.
J Protein Chem. 1997 Jul;16(5):469-79. doi: 10.1023/a:1026309410737.
3
A mass-spectrometric method for the estimation of the ratio of gamma-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin.一种用于估算蛋白质特定位点上γ-羧基谷氨酸与谷氨酸比例的质谱方法。应用于牛凝血酶原的N端区域。
Biochem J. 1980 Apr 1;187(1):239-43. doi: 10.1042/bj1870239.
4
Post-translational carboxylation of preprothrombin.凝血酶原前体的翻译后羧化作用。
Mol Cell Biochem. 1981 Aug 11;38 Spec No(Pt 1):77-121. doi: 10.1007/BF00235690.
5
beta-Hydroxyaspartic acid in vitamin K-dependent protein C.维生素K依赖蛋白C中的β-羟基天冬氨酸
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1802-6. doi: 10.1073/pnas.80.7.1802.
6
The N-terminal sequences of blood coagulation factor X1 and X2 light chains. Mass-spectrometric identification of twelve residues of gamma-carboxyglutamic acid in their vitamin K-dependent domains.凝血因子X1和X2轻链的N端序列。其维生素K依赖结构域中12个γ-羧基谷氨酸残基的质谱鉴定。
Biochem J. 1978 Nov 1;175(2):613-27. doi: 10.1042/bj1750613.

本文引用的文献

1
Experiments on the methylation and acetylation of wool, silk fibroin, collagen and gelatin.羊毛、丝素蛋白、胶原蛋白和明胶的甲基化和乙酰化实验。
Biochem J. 1944;38(2):171-8. doi: 10.1042/bj0380171.
2
THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.铜绿假单胞菌细胞色素C-551的氨基酸序列。
Biochem J. 1963 Nov;89(2):349-78. doi: 10.1042/bj0890349.
3
Preparation and properties of prothrombin.凝血酶原的制备与性质
J Biol Chem. 1959 Nov;234:2857-66.
4
[Amino acid determination on paper chromatograms].[纸色谱法测定氨基酸]
Hoppe Seylers Z Physiol Chem. 1957;309(4-6):219-20.
5
A simple procedure for separation of prothrombin and accelerator globulin from citrated human plasma.从枸橼酸化人血浆中分离凝血酶原和加速球蛋白的简单方法。
Proc Soc Exp Biol Med. 1953 Dec;84(3):636-40. doi: 10.3181/00379727-84-20737.
6
Studies towards the complete sequence determination of proteins by mass spectrometry; a rapid procedure for the successful permethylation of histidine containing peptides.
FEBS Lett. 1972 May 15;22(3):257-260. doi: 10.1016/0014-5793(72)80244-8.
7
Isolation and sme properties of prethrombin and autoprothrombin III.凝血酶原和自身凝血酶原III的分离及其某些特性
Arch Biochem Biophys. 1967 Aug;121(2):372-83. doi: 10.1016/0003-9861(67)90090-2.
8
Separation of dansyl-amino acids by polyamide layer chromatography.用聚酰胺薄层层析法分离丹磺酰氨基酸。
Biochim Biophys Acta. 1967 Feb 21;133(2):369-70. doi: 10.1016/0005-2795(67)90078-5.
9
Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines.菲醌作为精氨酸及其他单取代胍的分析试剂。
Biochim Biophys Acta. 1966 Dec 28;130(2):538-40. doi: 10.1016/0304-4165(66)90256-x.
10
Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.肽在纸上的电泳迁移率及其在酰胺基团测定中的应用。
Nature. 1966 Aug 6;211(5049):591-3. doi: 10.1038/211591a0.

凝血酶原N端区域新氨基酸（γ-羧基谷氨酸）十个残基的质谱鉴定及序列定位

Mass-spectrometric identification and sequence location of the ten residues of the new amino acid (gamma-Carboxyglutamic acid) in the N-terminal region of prothrombin.

作者信息

Morris H R, Dell A

出版信息

Biochem J. 1976 Mar 1;153(3):663-79. doi: 10.1042/bj1530663.

DOI:10.1042/bj1530663
PMID:942379
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172636/
Abstract

The detailed mass-spectrometric evidence for our original findings [Magnusson et al. (1974) FEBS Lett. 44, 189-193] of ten gamma-carboxyglutamic acid residues in the N-terminal calcium-binding polypeptide of prothrombin is presented. The identification and sequence location of gamma-carboxyglutamic acid was made by electron-impact and field-desorption studies on acetyl permethyl peptide derivatives, and on the free amino acid. Details of the derivatives formed, and how this new amino acid may be easily recognized and sequenced from the mass spectrum, are given as a basis for future work.

摘要

本文提供了详细的质谱分析证据，以证明我们最初的发现[Magnusson等人（1974年），《欧洲生物化学学会联合会快报》44卷，第189 - 193页]：凝血酶原N端钙结合多肽中存在十个γ-羧基谷氨酸残基。通过对乙酰基全甲基化肽衍生物和游离氨基酸进行电子轰击和场解吸研究，确定了γ-羧基谷氨酸的身份及其在序列中的位置。文中给出了所形成衍生物的详细信息，以及如何从质谱中轻松识别和测序这种新氨基酸，作为未来工作的基础。