凝血因子X1和X2轻链的N端序列。其维生素K依赖结构域中12个γ-羧基谷氨酸残基的质谱鉴定。
The N-terminal sequences of blood coagulation factor X1 and X2 light chains. Mass-spectrometric identification of twelve residues of gamma-carboxyglutamic acid in their vitamin K-dependent domains.
作者信息
Thøgersen H C, Petersen T E, Sottrup-Jensen L, Magnusson S, Morris H R
出版信息
Biochem J. 1978 Nov 1;175(2):613-27. doi: 10.1042/bj1750613.
Abstract
Peptides (residues 1-42) (bovine prothrombin numbering) from bovine Factor X1 and X2 have been separately purified and digested before mass-spectrometric sequence assignment and identification of gamma-carboxyglutamic acid. N-terminal sequence was found to be identical, and 12 residues of gamma-carboxyglutamic acid were unambiguously identified. The new data give conclusive evidence for the N-terminal primary structure of bovine Factor X and extend present knowledge to show (i) unambiguous assignment of gamma-carboxyglutamic acid residues, including a previously unreported residue of gamma-carboxyglutamic acid at position 40 in both Factor X1 and X2, (ii) the physical difference between Factors X1 and X2 is not due to either different amino acid sequences or different gamma-carboxyglutamic acid contents of the N-terminal 42 residues.
摘要
来自牛因子X1和X2的肽段（残基1 - 42）（牛凝血酶原编号）在进行质谱序列分配和γ-羧基谷氨酸鉴定之前，已分别进行了纯化和消化。发现其N端序列相同，并且明确鉴定出了12个γ-羧基谷氨酸残基。这些新数据为牛因子X的N端一级结构提供了确凿证据，并扩展了现有知识，表明：（i）γ-羧基谷氨酸残基的明确分配，包括因子X1和X2中第40位以前未报道的γ-羧基谷氨酸残基；（ii）因子X1和X2之间的物理差异并非由于N端42个残基的氨基酸序列不同或γ-羧基谷氨酸含量不同。
相似文献
1
The N-terminal sequences of blood coagulation factor X1 and X2 light chains. Mass-spectrometric identification of twelve residues of gamma-carboxyglutamic acid in their vitamin K-dependent domains.凝血因子X1和X2轻链的N端序列。其维生素K依赖结构域中12个γ-羧基谷氨酸残基的质谱鉴定。
Biochem J. 1978 Nov 1;175(2):613-27. doi: 10.1042/bj1750613.
2
3
A mass-spectrometric method for the estimation of the ratio of gamma-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin.一种用于估算蛋白质特定位点上γ-羧基谷氨酸与谷氨酸比例的质谱方法。应用于牛凝血酶原的N端区域。
Biochem J. 1980 Apr 1;187(1):239-43. doi: 10.1042/bj1870239.
4
[3H]diborane reduction of vitamin K-dependent calcium-binding proteins. Identification of a unique amino acid.
J Biol Chem. 1975 Apr 25;250(8):2968-72.
5
Isolation and characterization of vitamin K-dependent region of bovine blood clotting factor X.牛凝血因子X维生素K依赖区的分离与鉴定
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1281-5. doi: 10.1073/pnas.72.4.1281.
6
7
Distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins.γ-羧基谷氨酸残基在部分羧化的人凝血酶原中的分布
J Biol Chem. 1986 Feb 5;261(4):1624-8.
8
Metal binding sites of a gamma-carboxyglutamic acid-rich fragment of bovine prothrombin.
J Biol Chem. 1979 Dec 25;254(24):12521-30.
9
Identification of gamma-carboxyglutamic acid residues in bovine factors IX and X, and in a new vitamin K-dependent protein.
FEBS Lett. 1976 Oct 1;68(2):293-6. doi: 10.1016/0014-5793(76)80456-5.
10
Mass-spectrometric identification and sequence location of the ten residues of the new amino acid (gamma-Carboxyglutamic acid) in the N-terminal region of prothrombin.凝血酶原N端区域新氨基酸（γ-羧基谷氨酸）十个残基的质谱鉴定及序列定位
Biochem J. 1976 Mar 1;153(3):663-79. doi: 10.1042/bj1530663.
引用本文的文献
1
A novel geometry mass spectrometer, the Q-TOF, for low-femtomole/attomole-range biopolymer sequencing.
J Protein Chem. 1997 Jul;16(5):469-79. doi: 10.1023/a:1026309410737.
2
A mass-spectrometric method for the estimation of the ratio of gamma-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin.一种用于估算蛋白质特定位点上γ-羧基谷氨酸与谷氨酸比例的质谱方法。应用于牛凝血酶原的N端区域。
Biochem J. 1980 Apr 1;187(1):239-43. doi: 10.1042/bj1870239.
3
Post-translational carboxylation of preprothrombin.凝血酶原前体的翻译后羧化作用。
Mol Cell Biochem. 1981 Aug 11;38 Spec No(Pt 1):77-121. doi: 10.1007/BF00235690.
4
Characterization of a cDNA coding for human factor X.编码人凝血因子X的cDNA的特性分析
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3699-702. doi: 10.1073/pnas.81.12.3699.
5
Gamma-carboxyglutamic acid.γ-羧基谷氨酸
Mol Cell Biochem. 1981 Sep 25;39:191-207. doi: 10.1007/BF00232574.
6
Disulphide bridges of bovine factor X.牛凝血因子X的二硫键
Biochem J. 1987 Aug 1;245(3):887-91. doi: 10.1042/bj2450887.
7
Comparison of amino acid sequence of bovine coagulation Factor IX (Christmas Factor) with that of other vitamin K-dependent plasma proteins.牛凝血因子IX（克里斯马斯因子）与其他维生素K依赖血浆蛋白的氨基酸序列比较。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4990-4. doi: 10.1073/pnas.76.10.4990.
本文引用的文献
1
2
THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.铜绿假单胞菌细胞色素C-551的氨基酸序列。
Biochem J. 1963 Nov;89(2):349-78. doi: 10.1042/bj0890349.
3
NOMENCLATURE of blood clotting factors; four factors, their characterization and international number.血液凝血因子的命名；四种因子、其特性及国际编号
J Am Med Assoc. 1959 May 16;170(3):325-8.
4
[Amino acid determination on paper chromatograms].[纸色谱法测定氨基酸]
Hoppe Seylers Z Physiol Chem. 1957;309(4-6):219-20.
5
Studies towards the complete sequence determination of proteins by mass spectrometry; a rapid procedure for the successful permethylation of histidine containing peptides.
FEBS Lett. 1972 May 15;22(3):257-260. doi: 10.1016/0014-5793(72)80244-8.
6
Separation of dansyl-amino acids by polyamide layer chromatography.用聚酰胺薄层层析法分离丹磺酰氨基酸。
Biochim Biophys Acta. 1967 Feb 21;133(2):369-70. doi: 10.1016/0005-2795(67)90078-5.
7
Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines.菲醌作为精氨酸及其他单取代胍的分析试剂。
Biochim Biophys Acta. 1966 Dec 28;130(2):538-40. doi: 10.1016/0304-4165(66)90256-x.
8
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
9
Studies on bovine factor X. II. Characterization of purified factor X. Observations on some alterations in zone electrophoretic and chromatographic behavior occurring during purification.牛凝血因子X的研究。II. 纯化的凝血因子X的特性。关于纯化过程中区域电泳和色谱行为某些变化的观察。
Biochemistry. 1968 Dec;7(12):4506-17. doi: 10.1021/bi00852a047.
10
Mass spectrometry of permethylated peptide derivatives; extension of the technique to peptides containing arginine or methionine.
Biochem Biophys Res Commun. 1968 Aug 13;32(3):519-25. doi: 10.1016/0006-291x(68)90693-1.
Zotero 插件