Drapkin R L, Adreeff M, Koziner B, Strife A, Wisniewski D, Darzynkiewicz Z, Melamed M R, Clarkson B
Am J Hematol. 1979;7(2):163-72. doi: 10.1002/ajh.2830070209.
Normal human peripheral blood cells were separated into different populations based upon isopycnic sedimentation, E rosetting, and EAC rosetting. Each population was characterized according to morphology, surface markers, granulocytic colony formation in semi-solid media, and stainable RNA content by acridine orange (AO) flow cytometry. These techniques enrich for a population of cells that is characterized by a lymphoid morphology, a high granulocytic-macrophage progenitor cell cloning efficiency, a lack of surface markers, and a high stainable RNA content not found in the other two populations of peripheral blood lymphocytes (T cells and B cells). The stainable RNA content serves as a new metabolic marker for the population of cells in which the preponderance of granulocytic progenitor cells reside.
正常人类外周血细胞根据等密度沉降、E 玫瑰花结形成和 EAC 玫瑰花结形成被分离成不同的群体。每个群体根据形态学、表面标志物、半固体培养基中的粒细胞集落形成以及吖啶橙（AO）流式细胞术检测的可染色 RNA 含量进行表征。这些技术富集了一群细胞，其特征为具有淋巴细胞形态、高粒细胞 - 巨噬细胞祖细胞克隆效率、缺乏表面标志物以及在其他两群外周血淋巴细胞（T 细胞和 B 细胞）中未发现的高可染色 RNA 含量。可染色 RNA 含量可作为粒细胞祖细胞占优势的细胞群体的一种新的代谢标志物。
