Andreeff M, Beck J D, Darzynkiewicz Z, Traganos F, Gupta S, Melamed M R, Good R A
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1938-42. doi: 10.1073/pnas.75.4.1938.
Human peripheral blood lymphocytes are stained with the metachromatic dye acridine orange and the fluorescence of individual cells is measured by flow cytometry. The relative content of stainable RNA per cell is estimated by comparison with RNase-treated cells. Non-T and T lymphocytes have different mean quantities of RNA per cell, and these classes exhibit different distributions of RNA content. Non-T cells have a unimodal distribution with a sharp peak and exponential distribution towards higher RNA values. T cells have a bimodal distribution with two separate peaks. When T cells having receptors for IgG (Tgamma cells) and IgM (Tmu cells) are separated, each of these cell populations displays a unimodal distribution. Of these three lymphocyte subpopulations, Tgamma cells have the lowest content of RNA per cell. Non-T cells have slightly higher RNA content than Tgamma, and Tmu cells have twice as much RNA as Tgamma cells. The RNA content, which surely relates to the different functions of these lymphocyte subpopulations, may also be a useful marker for rapidly distinguishing the lymphocyte subpopulations.
用异染染料吖啶橙对人外周血淋巴细胞进行染色，然后通过流式细胞术测量单个细胞的荧光。通过与经核糖核酸酶处理的细胞进行比较，估算每个细胞中可染色RNA的相对含量。非T淋巴细胞和T淋巴细胞每个细胞的RNA平均含量不同，并且这两类细胞呈现出不同的RNA含量分布。非T细胞具有单峰分布，有一个尖锐的峰值，并向较高RNA值呈指数分布。T细胞具有双峰分布，有两个分开的峰。当分离出具有IgG受体的T细胞（Tγ细胞）和具有IgM受体的T细胞（Tμ细胞）时，这些细胞群体中的每一个都呈现单峰分布。在这三个淋巴细胞亚群中，Tγ细胞每个细胞的RNA含量最低。非T细胞的RNA含量比Tγ细胞略高，而Tμ细胞的RNA含量是Tγ细胞的两倍。RNA含量肯定与这些淋巴细胞亚群的不同功能有关，它也可能是快速区分淋巴细胞亚群的一个有用标记。
