Krasnikov B F, Kuzminova A E, Zorov D B
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
FEBS Lett. 1997 Dec 8;419(1):137-40. doi: 10.1016/s0014-5793(97)01450-6.
The oxygen dependence of the mitochondria permeability transition pore was under study in non-respiring rat liver mitochondria. Oxygen in the medium was depleted by saturation of the incubation medium with N2 and spontaneously by mitochondrial respiration followed by the addition of glucose/glucose oxidase. After the anaerobic state had been reached, ferricyanide has been added to support succinate-driven energization in the absence of oxygen. In the other set of the experiments KCN was added to block operation of the respiratory chain under aerobic conditions. Again, ferricyanide was added as an electron acceptor. Superoxide dismutase was added to trap superoxide anion radicals. Under either hypoxic conditions or in the presence of cyanide, calcium ions were shown to induce the permeability transition. The concentration of Ca2+ required was lower than under conditions of active respiration. In both cases, the transition was prevented by cyclosporine A.
在非呼吸状态的大鼠肝脏线粒体中研究线粒体通透性转换孔的氧依赖性。通过用N2饱和孵育培养基并随后添加葡萄糖/葡萄糖氧化酶，使培养基中的氧自然地被线粒体呼吸耗尽。达到厌氧状态后，加入铁氰化物以在无氧条件下支持琥珀酸驱动的能量化。在另一组实验中，加入KCN以阻断有氧条件下呼吸链的运作。同样，加入铁氰化物作为电子受体。加入超氧化物歧化酶以捕获超氧阴离子自由基。在缺氧条件或存在氰化物的情况下，钙离子均显示可诱导通透性转换。所需的Ca2+浓度低于活跃呼吸条件下的浓度。在这两种情况下，环孢素A均可阻止转换。
