Hayashi T, Matsufuji S, Hayashi S
Department of Biochemistry II, The Jikei University School of Medicine, Tokyo, Japan.
Gene. 1997 Dec 12;203(2):131-9. doi: 10.1016/s0378-1119(97)00504-0.
Antizyme is a polyamine-inducible protein involved in feedback regulation of cellular polyamine levels. Recently, we isolated genomic clones for the human antizyme gene and determined its chromosomal location (Matsufuji et al., Genomics 38 (1996) 112-114). In the present study, we report complete nucleotide sequence and organization of the human antizyme gene. The organizations of human and rat genes are very similar, but their introns show divergency in terms of the length and nucleotide sequence. Luciferase reporter assay revealed that the 5'-flanking region of the human gene had a strong transcriptional activity in NIH-3T3 with and without addition of spermidine. The promoter was also effective in transfected COS7 and HeLa cells. A 223-bp region at the proximity of the transcriptional start points carries several regulatory sequence motifs including a TATA box, CAAT boxes and GC boxes, and was shown to be important for the strong transcriptional activity.
抗酶是一种多胺诱导蛋白，参与细胞多胺水平的反馈调节。最近，我们分离了人类抗酶基因的基因组克隆，并确定了其染色体定位（松藤等人，《基因组学》38卷（1996年）112 - 114页）。在本研究中，我们报告了人类抗酶基因的完整核苷酸序列和结构。人类和大鼠基因的结构非常相似，但它们的内含子在长度和核苷酸序列方面存在差异。荧光素酶报告基因检测显示，人类基因的5'侧翼区在添加和不添加亚精胺的情况下，在NIH - 3T3细胞中都具有很强的转录活性。该启动子在转染的COS7细胞和HeLa细胞中也有效。转录起始点附近的一个223 bp区域含有几个调控序列基序，包括一个TATA盒、CAAT盒和GC盒，并且已证明该区域对于强转录活性很重要。
