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编码依赖DNA的蛋白激酶催化亚基的信使核糖核酸在非洲爪蟾细胞中广泛表达。

mRNA encoding the catalytic subunit of DNA-dependent protein kinase is widely expressed in Xenopus cells.

作者信息

Labhart P

机构信息

The Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, CA 92037, USA.

出版信息

Gene. 1997 Dec 12;203(2):235-40. doi: 10.1016/s0378-1119(97)00498-8.

DOI:10.1016/s0378-1119(97)00498-8
PMID:9426255
Abstract

Here, the sequence of a Xenopus laevis cDNA encoding the 640 carboxy-terminal amino acids of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is reported. The predicted Xenopus protein segment is 65% identical to the human counterpart. Northern blot analysis indicates that Xenopus DNA-PKcs is encoded by an approx. 13000 nt transcript. DNA-PKcs mRNA is widely expressed in adult tissues as well as in oocytes and embryos. It is also shown that outside the conserved kinase domain, Xenopus DNA-PKcs bears significant similarities to hypothetical 420.8 and 433.2 kDa proteins in yeast species.

摘要

本文报道了非洲爪蟾（Xenopus laevis）编码DNA依赖性蛋白激酶（DNA-PKcs）催化亚基640个羧基末端氨基酸的cDNA序列。预测的非洲爪蟾蛋白片段与人类对应片段的同源性为65%。Northern印迹分析表明，非洲爪蟾DNA-PKcs由一个约13000 nt的转录本编码。DNA-PKcs mRNA在成年组织以及卵母细胞和胚胎中广泛表达。研究还表明，在保守的激酶结构域之外，非洲爪蟾DNA-PKcs与酵母中的假想420.8 kDa和433.2 kDa蛋白具有显著相似性。

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Exogenously expressed human Ku70 stabilizes Ku80 in Xenopus oocytes and induces heterologous DNA-PK catalytic activity.外源性表达的人 Ku70 稳定 Xenopus 卵母细胞中的 Ku80，并诱导异源 DNA-PK 催化活性。
Mol Cell Biochem. 2010 May;338(1-2):291-8. doi: 10.1007/s11010-009-0363-3. Epub 2010 Jan 3.
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Ku-dependent nonhomologous DNA end joining in Xenopus egg extracts.
非洲爪蟾卵提取物中依赖Ku蛋白的非同源DNA末端连接
Mol Cell Biol. 1999 Apr;19(4):2585-93. doi: 10.1128/MCB.19.4.2585.
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Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids.Ku蛋白与DNA依赖性蛋白激酶催化亚基与核酸的相互作用。
Nucleic Acids Res. 1998 Apr 1;26(7):1551-9. doi: 10.1093/nar/26.7.1551.