Kolokol'chikova E G, Pal'tsyn A A
Biull Eksp Biol Med. 1979 Nov;88(11):617-21.
The RNA and protein synthesis in mouse fibroblasts was studied on the 3d and 7th day of wound healing by electron-microscopic autoradiography with double-labeling technique. It was shown that the ratio between the protein synthesis in cytoplasm and the RNA synthesis in the nucleus of some cells significantly differed resulting in relative predominance of one of the two processes studied in the fibroblasts during both terms of wound healing. On an average the fibroblasts were characterized by a higher level of the protein synthesis on the 3d day of wound healing. This average high level was provided by an increase in the fibroblasts, in which the protein synthesis prevailed over the RNA one.
采用双标记技术的电子显微镜放射自显影法,在伤口愈合的第3天和第7天对小鼠成纤维细胞中的RNA和蛋白质合成进行了研究。结果表明,某些细胞的细胞质中蛋白质合成与细胞核中RNA合成的比例存在显著差异,导致在伤口愈合的两个阶段,成纤维细胞中所研究的两个过程之一相对占优势。平均而言,在伤口愈合的第3天,成纤维细胞的蛋白质合成水平较高。这种平均高水平是由成纤维细胞数量增加所致,其中蛋白质合成超过RNA合成。